WB
Western blot analysis of various lysates, using KD Validated] PKA C-alpha Mouse mAb at 1:10000 dlilution. Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 20s.WB
Western blot analysis of lysates from wld type(T) and PKA C-alpha (PRKACA)knockdown (KD)293T cells, using KD Validated] PKA C-alpha (PRKACA) Mouse mAb at 1:10000 dilution. Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time:20s.ICC/IF
Immunofluorescence analysis of BALB- 3T3 cells using [KD Validated] PKA C-alpha (PRKACA) Mouse mAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.ICC/IF
Immunofluorescence analysis of MCF7 cells using IKD Validated] PKA C -alpha (PRKACA) Mouse mAb at dilution of 1:100 (40x lens) Blue: DAPI for nuclear staining.| Product Name | PKA C-alpha (PRKACA) Mouse mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein (or fragment).This information is considered to be commercially sensitive. |
| Clonality | Monoclonal |
|---|---|
| Isotype | Ig2b |
| Host Species | Mouse |
| Tested Applications | ELISAICC/IFWB |
| WB:1:10000 - 1:40000 ICC/IF:1:50 - 1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PRKACA |
|---|---|
| Gene Synonyms | CAFD1 PKACA PPNAD4 |
| Gene Full Name | protein kinase cAMP-activated catalytic subunit alpha |
| Gene Summary | This gene encodes one of the catalytic subunits of protein kinase A, which exists as a tetrameric holoenzyme with two regulatory subunits and two catalytic subunits, in its inactive form. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. cAMP-dependent phosphorylation of proteins by protein kinase A is important to many cellular processes, including differentiation, proliferation, and apoptosis. Constitutive activation of this gene caused either by somatic mutations, or genomic duplications of regions that include this gene, have been associated with hyperplasias and adenomas of the adrenal cortex and are linked to corticotropin-independent Cushing's syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms. Tissue-specific isoforms that differ at the N-terminus have been described, and these isoforms may differ in the post-translational modifications that occur at the N-terminus of some isoforms. [provided by RefSeq, Jan 2015] |
| Alternative Names | CAFD1 PKACA PPNAD4 A) |
| Molecular Weight(MW) | 41kDa（Observed band size:40kDa） |
| Cellular Localization | Cell membrane,Cell projection,Cilium,Cytoplasm,Cytoplasmic vesicle |
WB
Western blot analysis of various lysates, using KD Validated] PKA C-alpha Mouse mAb at 1:10000 dlilution. Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 20s.
WB
Western blot analysis of lysates from wld type(T) and PKA C-alpha (PRKACA)knockdown (KD)293T cells, using KD Validated] PKA C-alpha (PRKACA) Mouse mAb at 1:10000 dilution. Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time:20s.
ICC/IF
Immunofluorescence analysis of BALB- 3T3 cells using [KD Validated] PKA C-alpha (PRKACA) Mouse mAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ICC/IF
Immunofluorescence analysis of MCF7 cells using IKD Validated] PKA C -alpha (PRKACA) Mouse mAb at dilution of 1:100 (40x lens) Blue: DAPI for nuclear staining.| Application Notes | WB:1:10000 - 1:40000 ICC/IF:1:50 - 1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3. |
