WB
Western blot analysis of RAB35 on different lysates with Rabbit anti-RAB35 antibody at 1/5,000 dilution. Lysates/proteins at 15 µg/Lane. Exposure time: 20 seconds; Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: 1/5,000 in primary antibody dilution buffer, overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP, 1/50,000 in 5% NFDM/TBST, 1 hour at room temperatureICC/IF
Application: Immunocytochemistry (IF-cell) Species: Human Sample: Jurkat (Human T-lymphoblastic cells) Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature. Antibody dilution buffer: 1% BSA in PBST. Primary antibody: 1/50, overnight at 4°C. Secondary antibody: Goat Anti-Rabbit IgG (488), 45 minutes at room temperature. Counterstain: Beta tubulin (Red), 1/100, overnight at 4℃. The nuclear counterstain was DAPI (Blue).ICC/IF
Application: Immunocytochemistry (IF-cell) Species: Mouse Sample: RAW264.7 (Mouse monocytic macrophage leukemia cell) Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature. Antibody dilution buffer: 1% BSA in PBST. Primary antibody:1/100, overnight at 4°C. Secondary antibody: Goat Anti-Rabbit IgG ( 488), 45 minutes at room temperature. Counterstain: Beta tubulin (Red), 1/100, overnight at 4℃. The nuclear counterstain was DAPI (Blue).ICC/IF
Application: Immunocytochemistry (IF-cell) Species: Rat Sample: PC-12 (Rat pheochromocytoma cell (undifferentiated)) Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature. Antibody dilution buffer: 1% BSA in PBST. Primary antibody: 1/100, overnight at 4°C. Secondary antibody: Goat Anti-Rabbit IgG (488), 45 minutes at room temperature. Counterstain: Beta tubulin (Red), 1/100, overnight at 4℃. The nuclear counterstain was DAPI (Blue).ICC/IF
Application: Immunohistochemistry (IHC-P) Species: Human Tissue: Breast cancer Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: 1/1,000, 1 hour at room temperature. Secondary antibody: 20 minutes at room temperature.IHC
Application: Immunohistochemistry (IHC-P) Species: Human Tissue: Breast cancer Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: 1/1,000, 1 hour at room temperature. Secondary antibody: 20 minutes at room temperature.FC
Application: Flow Cytometry (Intra) Species: Human Sample: Jurkat (Human T-lymphoblastic cells) Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 0.1% Tween-20, 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature. Antibody dilution buffer: 1x PBS. Primary antibody: RAB35 Recombinant Rabbit Monoclonal Antibody (1/1,000, Red) compared with Rabbit IgG Isotype Control (Green), 15 minutes at room temperature. Secondary antibody: Goat Anti-Rabbit IgG ( 488), 15 minutes at room temperature.| Product Name | RAB35 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within human RAB35 aa 1-201. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIHCWB |
| WB:1:5000 ICC/IF:1:50-1:100 IHC:1:1000 FC:1:1000 |
|
| Species Reactivity | HumanMonkeyMousePigRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | RAB35 |
|---|---|
| Gene Synonyms | RAY H-ray RAB1C |
| Gene Full Name | RAB35, member RAS oncogene family |
| Gene Summary | Enables GTPase activity and anion binding activity. Involved in several processes, including endosomal transport; plasma membrane to endosome transport; and protein localization to endosome. Located in several cellular components, including clathrin-coated endocytic vesicle; clathrin-coated pit; and intercellular bridge. [provided by Alliance of Genome Resources, Apr 2025] |
| Alternative Names | GTP binding protein RAY antibody GTP-binding protein RAY antibody H ray antibody OTTHUMP00000240256 antibody OTTHUMP00000240257 antibody OTTHUMP00000240258 antibody OTTHUMP00000240259 antibody RAB1C antibody Rab35 antibody RAB35 member RAS oncogene family antibody GTP binding protein RAY antibody GTP-binding protein RAY antibody H ray antibody OTTHUMP00000240256 antibody OTTHUMP00000240257 antibody OTTHUMP00000240258 antibody OTTHUMP00000240259 antibody RAB1C antibody Rab35 antibody RAB35 member RAS oncogene family antibody RAB35_HUMAN antibody Ras related protein rab 1c (GTP binding protein ray) antibody Ras related protein Rab 1C antibody Ras related protein Rab35 antibody Ras-related protein Rab-1C antibody Ras-related protein Rab-35 antibody RAY antibody |
| Molecular Weight(MW) | 23 kDa |
| Cellular Localization | Cell membrane, Membrane, clathrin-coated pit, Cytoplasmic vesicle, clathrin-coated vesicle, Endosome, Melanosome. |
WB
Western blot analysis of RAB35 on different lysates with Rabbit anti-RAB35 antibody at 1/5,000 dilution. Lysates/proteins at 15 µg/Lane. Exposure time: 20 seconds; Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: 1/5,000 in primary antibody dilution buffer, overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP, 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
ICC/IF
Application: Immunocytochemistry (IF-cell) Species: Human Sample: Jurkat (Human T-lymphoblastic cells) Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature. Antibody dilution buffer: 1% BSA in PBST. Primary antibody: 1/50, overnight at 4°C. Secondary antibody: Goat Anti-Rabbit IgG (488), 45 minutes at room temperature. Counterstain: Beta tubulin (Red), 1/100, overnight at 4℃. The nuclear counterstain was DAPI (Blue).
ICC/IF
Application: Immunocytochemistry (IF-cell) Species: Mouse Sample: RAW264.7 (Mouse monocytic macrophage leukemia cell) Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature. Antibody dilution buffer: 1% BSA in PBST. Primary antibody:1/100, overnight at 4°C. Secondary antibody: Goat Anti-Rabbit IgG ( 488), 45 minutes at room temperature. Counterstain: Beta tubulin (Red), 1/100, overnight at 4℃. The nuclear counterstain was DAPI (Blue).
ICC/IF
Application: Immunocytochemistry (IF-cell) Species: Rat Sample: PC-12 (Rat pheochromocytoma cell (undifferentiated)) Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature. Antibody dilution buffer: 1% BSA in PBST. Primary antibody: 1/100, overnight at 4°C. Secondary antibody: Goat Anti-Rabbit IgG (488), 45 minutes at room temperature. Counterstain: Beta tubulin (Red), 1/100, overnight at 4℃. The nuclear counterstain was DAPI (Blue).
ICC/IF
Application: Immunohistochemistry (IHC-P) Species: Human Tissue: Breast cancer Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: 1/1,000, 1 hour at room temperature. Secondary antibody: 20 minutes at room temperature.
IHC
Application: Immunohistochemistry (IHC-P) Species: Human Tissue: Breast cancer Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: 1/1,000, 1 hour at room temperature. Secondary antibody: 20 minutes at room temperature.
FC
Application: Flow Cytometry (Intra) Species: Human Sample: Jurkat (Human T-lymphoblastic cells) Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 0.1% Tween-20, 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature. Antibody dilution buffer: 1x PBS. Primary antibody: RAB35 Recombinant Rabbit Monoclonal Antibody (1/1,000, Red) compared with Rabbit IgG Isotype Control (Green), 15 minutes at room temperature. Secondary antibody: Goat Anti-Rabbit IgG ( 488), 15 minutes at room temperature.| Application Notes | WB:1:5000 ICC/IF:1:50-1:100 IHC:1:1000 FC:1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage Buffer | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
