Application
RAB35 Antibody (C-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the RAB35 antibody detected the RAB35 protein (arrow).| Product Name | RAB35 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RAB35; RAB1C; RAY; Ras-related protein Rab-35; GTP-binding protein RAY; Ras-related protein Rab-1C |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | RAB1C RAY |
|---|---|
| Alternative Names | RAB35 RAB1C RAY Ras-related protein Rab-35 GTP-binding protein RAY Ras-related protein Rab-1C |
| Molecular Weight(MW) | 23025 Da |
| Function | In the process of endocytosis, essential rate-limiting regulator of a fast recycling pathway back to the plasma membrane During cytokinesis, required for the postfurrowing terminal steps, namely for intercellular bridge stability and abscission, possibly by controlling phosphatidylinositol 4,5-bis phosphate (PIP2) and SEPT2 localization at the intercellular bridge |
| Tissue Specificity | This RAB35 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 170-200 amino acids from the C-terminal region of human RAB35. |
| Cellular Localization | Cell membrane; Lipid-anchor; Cytoplasmic side (Potential). Membrane, clathrin-coated pit. Cytoplasmic vesicle, clathrin-coated vesicle. Endosome. Melanosome Note=Present on sorting endosomes and endosomal tubules. Tends to be enriched in PIP2-positive cell membrane domains. During mitosis, associated with the plasma membrane and present at the ingressing furrow during early cytokinesis as well as at the intercellular bridge later during cytokinesis. Identified by mass spectrometry in melanosome fractions from stage I to stage IV |
| Entrez Gene | 11021 |
|---|---|
| Nucleotide Accession | NP_001161078.1 |
Application
RAB35 Antibody (C-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the RAB35 antibody detected the RAB35 protein (arrow).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
