WB
Western blot analysis of OTOF using anti-OTOF antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-OTOF antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.IHC
IHC analysis of OTOF using anti-OTOF antibody. OTOF was detected in a paraffin-embedded section of mouse brain tissue. The tissue section was incubated with rabbit anti-OTOF Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
IHC analysis of OTOF using anti-OTOF antibody. OTOF was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-OTOF Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.| Product Name | Rabbit polyclonal antibody to OTOF |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human Otoferlin, identical to the related mouse and rat sequences. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:500-1:2000 IHC:1:50-1:400 |
|
| Species Reactivity | MouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | OTOF |
|---|---|
| Gene Synonyms | AUNB1 DFNB6 DFNB9 NSRD9 FER1L2 |
| Gene Full Name | otoferlin |
| Gene Summary | Mutations in this gene are a cause of neurosensory nonsyndromic recessive deafness, DFNB9. The short form of the encoded protein has 3 C2 domains, a single carboxy-terminal transmembrane domain found also in the C. elegans spermatogenesis factor FER-1 and human dysferlin, while the long form has 6 C2 domains. The homology suggests that this protein may be involved in vesicle membrane fusion. Several transcript variants encoding multiple isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| Alternative Names | otoferlin AUNB1 DFNB6 DFNB9 FER1L2 NSRD9 OTOF |
| Molecular Weight(MW) | 227 kDa |
| Cellular Localization | Cell membrane,Cell projection,Cytoplasmic vesicle,Endoplasmic reticulum,Golgi apparatus,Membrane,Synapse. |
WB
Western blot analysis of OTOF using anti-OTOF antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-OTOF antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
IHC
IHC analysis of OTOF using anti-OTOF antibody. OTOF was detected in a paraffin-embedded section of mouse brain tissue. The tissue section was incubated with rabbit anti-OTOF Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of OTOF using anti-OTOF antibody. OTOF was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-OTOF Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt，-20℃ as supplied. |
| Storage Buffer | 500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
