WB
Various whole cell lysates were separated by 4-8% SDS-PAGE, and the membrane was blotted with anti-VEGFR2 (Phospho Tyr1175) antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody.| Product Name | Flk-1/VEGFR2 (Phospho Tyr1175) Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Modification | p-Tyr1175 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG,K |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:2000-1:10000 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | KDR |
|---|---|
| Gene Synonyms | FLK1 CD309 VEGFR VEGFR2 |
| Gene Full Name | kinase insert domain receptor |
| Gene Summary | Vascular endothelial growth factor (VEGF) is a major growth factor for endothelial cells. This gene encodes one of the two receptors of the VEGF. This receptor, known as kinase insert domain receptor, is a type III receptor tyrosine kinase. It functions as the main mediator of VEGF-induced endothelial proliferation, survival, migration, tubular morphogenesis and sprouting. The signalling and trafficking of this receptor are regulated by multiple factors, including Rab GTPase, P2Y purine nucleotide receptor, integrin alphaVbeta3, T-cell protein tyrosine phosphatase, etc.. Mutations of this gene are implicated in infantile capillary hemangiomas. [provided by RefSeq, May 2009] |
| Alternative Names | KDR FLK1 VEGFR2 Vascular endothelial growth factor receptor 2 VEGFR-2 Fetal liver kinase 1 FLK-1 Kinase insert domain receptor KDR Protein-tyrosine kinase receptor flk-1 CD antigen CD309 |
| Molecular Weight(MW) | 152 kDa(Observed band size: 250 kDa) |
| Cellular Localization | Cell junction . Endoplasmic reticulum . Cell membrane . Localized with RAP1A at cell-cell junctions (By similarity) . Colocalizes with ERN1 and XBP1 in the endoplasmic reticulum in endothelial cells in a vascular endothelial growth factor (VEGF) -dependent manner (PubMed:23529610) . .; [Isoform 1]: Cell membrane; Single-pass type I membrane protein. Cytoplasm. Nucleus. Cytoplasmic vesicle. Early endosome. Detected on caveolae-enriched lipid rafts at the cell surface. Is recycled from the plasma membrane to endosomes and back again. Phosphorylation triggered by VEGFA binding promotes internalization and subsequent degradation. VEGFA binding triggers internalization and translocation to the nucleus.; [Isoform 2]: Secreted .; [Isoform 3]: Secreted. |
WB
Various whole cell lysates were separated by 4-8% SDS-PAGE, and the membrane was blotted with anti-VEGFR2 (Phospho Tyr1175) antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody.| Application Notes | WB:1:2000-1:10000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year (Do not lower than -25°C). |
| Storage Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA. |
