WB
Western blot analysis of VEGFR2/KDR using anti-VEGFR2/KDR antibody. Lane 1: recombinant mouse VEGFR2 Protein 1ng. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-VEGFR2/KDR antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.IHC
IHC analysis of VEGFR2/KDR using anti-VEGFR2/KDR antibody. VEGFR2/KDR was detected in a paraffin-embedded section of mouse kidney tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-VEGFR2/KDR Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.IHC
IHC analysis of VEGFR2/KDR using anti-VEGFR2/KDR antibody. VEGFR2/KDR was detected in a paraffin-embedded section of rat kidney tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-VEGFR2/KDR Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.ICC/IF
ICC/IF analysis of VEGFR2/KDR using anti-VEGFR2/KDR antibody. VEGFR2/KDR was detected in an immunocytochemical section of NIH/3T3 cells. The section was incubated with rabbit anti-VEGFR2/KDR Antibody at a dilution of 1:100. Fluoro594-conjugated Anti-rabbit IgG Secondary Antibody (red) was used as secondary antibody. The section was counterstained with DAPI (Blue).FC
Flow Cytometry analysis of LLC cells using anti-VEGFR2/KDR antibody. Overlay histogram showing LLC cells stained with Anti-VEGFR2/KDR Antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-VEGFR2/KDR Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Product Name | Rabbit polyclonal antibody to VEGFR2/KDR |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E. coli-derived mouse VEGF Receptor 2 recombinant protein (Position: A20-L244). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 FC:1:50-1:200 |
|
| Species Reactivity | MouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | KDR |
|---|---|
| Gene Synonyms | FLK1 CD309 VEGFR VEGFR2 |
| Gene Full Name | kinase insert domain receptor |
| Gene Summary | Vascular endothelial growth factor (VEGF) is a major growth factor for endothelial cells. This gene encodes one of the two receptors of the VEGF. This receptor, known as kinase insert domain receptor, is a type III receptor tyrosine kinase. It functions as the main mediator of VEGF-induced endothelial proliferation, survival, migration, tubular morphogenesis and sprouting. The signalling and trafficking of this receptor are regulated by multiple factors, including Rab GTPase, P2Y purine nucleotide receptor, integrin alphaVbeta3, T-cell protein tyrosine phosphatase, etc.. Mutations of this gene are implicated in infantile capillary hemangiomas. [provided by RefSeq, May 2009] |
| Alternative Names | CD309 Fetal liver kinase 1 FLK 1 FLK1 KDR Kinase insert domain receptor VEGF Receptor 2 VEGFR VEGFR 2 VEGFR2 |
| Molecular Weight(MW) | 150 kDa(Observed band size: 180-250 kDa) |
| Cellular Localization | Cell junction,Cell membrane,Cytoplasm,Cytoplasmic vesicle,Endoplasmic reticulum,Endosome,Membrane,Nucleus,Secreted. |
WB
Western blot analysis of VEGFR2/KDR using anti-VEGFR2/KDR antibody. Lane 1: recombinant mouse VEGFR2 Protein 1ng. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-VEGFR2/KDR antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
IHC
IHC analysis of VEGFR2/KDR using anti-VEGFR2/KDR antibody. VEGFR2/KDR was detected in a paraffin-embedded section of mouse kidney tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-VEGFR2/KDR Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IHC
IHC analysis of VEGFR2/KDR using anti-VEGFR2/KDR antibody. VEGFR2/KDR was detected in a paraffin-embedded section of rat kidney tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-VEGFR2/KDR Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
ICC/IF
ICC/IF analysis of VEGFR2/KDR using anti-VEGFR2/KDR antibody. VEGFR2/KDR was detected in an immunocytochemical section of NIH/3T3 cells. The section was incubated with rabbit anti-VEGFR2/KDR Antibody at a dilution of 1:100. Fluoro594-conjugated Anti-rabbit IgG Secondary Antibody (red) was used as secondary antibody. The section was counterstained with DAPI (Blue).
FC
Flow Cytometry analysis of LLC cells using anti-VEGFR2/KDR antibody. Overlay histogram showing LLC cells stained with Anti-VEGFR2/KDR Antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-VEGFR2/KDR Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 FC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt，-20℃ as supplied. |
| Storage Buffer | 500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
