WB
Western blot analysis of KLB using anti-KLB antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-KLB antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.IHC
IHC analysis of KLB using anti-KLB antibody. KLB was detected in a paraffin-embedded section of mouse liver tissue. The tissue section was incubated with rabbit anti-KLB Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
IHC analysis of KLB using anti-KLB antibody. KLB was detected in a paraffin-embedded section of rat liver tissue. The tissue section was incubated with rabbit anti-KLB Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.| Product Name | Rabbit polyclonal antibody to KLB |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human KLB recombinant protein (Position: N484-R855). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:500-1:2000 IHC:1:50-1:400 |
|
| Species Reactivity | MouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | KLB |
|---|---|
| Gene Synonyms | BKL |
| Gene Full Name | klotho beta |
| Gene Summary | Enables fibroblast growth factor binding activity and fibroblast growth factor receptor binding activity. Predicted to be involved in carbohydrate metabolic process. Predicted to act upstream of or within positive regulation of MAPKKK cascade by fibroblast growth factor receptor signaling pathway and positive regulation of cell population proliferation. Predicted to be located in plasma membrane. [provided by Alliance of Genome Resources, Jul 2025] |
| Alternative Names | KLB Beta-klotho BKL BetaKlotho Klotho beta-like protein |
| Molecular Weight(MW) | 125 kDa(Observed band size:150 kDa) |
| Cellular Localization | Cell membrane,Membrane. |
WB
Western blot analysis of KLB using anti-KLB antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-KLB antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
IHC
IHC analysis of KLB using anti-KLB antibody. KLB was detected in a paraffin-embedded section of mouse liver tissue. The tissue section was incubated with rabbit anti-KLB Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of KLB using anti-KLB antibody. KLB was detected in a paraffin-embedded section of rat liver tissue. The tissue section was incubated with rabbit anti-KLB Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| Storage Buffer | 500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
