WB
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FOXA2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FOXA2.IHC
Immunohistochemical staining of paraffin-embedded Carcinoma of liver using anti-FOXA2 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.ICC/IF
Anti-FOXA2 mouse monoclonal antibody immunofluorescent staining of HeLa cells transiently transfected by pCMV6-ENTRY FOXA2.| Product Name | FOXA2 Mouse Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein expressed in E.coli corresponding to amino acids 300-458 of human FOXA2. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | FCICC/IFIHCWB |
| WB:1:1000 IHC:1:50 ICC/IF:1:50-1:100 FC:1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A/G |
| Gene Symbol | FOXA2 |
|---|---|
| Gene Synonyms | HNF3B TCF3B HNF-3-beta |
| Gene Full Name | forkhead box A2 |
| Gene Summary | This gene encodes a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific genes such as albumin and transthyretin, and they also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver. This gene has been linked to sporadic cases of maturity-onset diabetes of the young. Transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Oct 2008] |
| Alternative Names | HNF3B TCF3B |
| Molecular Weight(MW) | 48.1 kDa |
| Cellular Localization | Cytoplasm,Nucleus. |
WB
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FOXA2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FOXA2.
IHC
Immunohistochemical staining of paraffin-embedded Carcinoma of liver using anti-FOXA2 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.
ICC/IF
Anti-FOXA2 mouse monoclonal antibody immunofluorescent staining of HeLa cells transiently transfected by pCMV6-ENTRY FOXA2.| Application Notes | WB:1:1000 IHC:1:50 ICC/IF:1:50-1:100 FC:1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C as received. |
| Storage Buffer | PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
