WB
Western blot analysis of ANGPTL4 on different lysates with Rabbit anti-ANGPTL4 antibody at 1/2,000 dilution. Lysates/proteins at 20 µg/Lane. Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.| Product Name | ANGPTL4 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within human ANGPTL4 aa 107-406. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:2000 |
|
| Species Reactivity | HumanMonkey |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | ANGPTL4 |
|---|---|
| Gene Synonyms | NL2 ARP4 FIAF HARP PGAR HFARP TGQTL UNQ171 pp1158 |
| Gene Full Name | angiopoietin like 4 |
| Gene Summary | This gene encodes a glycosylated, secreted protein containing a C-terminal fibrinogen domain. The encoded protein is induced by peroxisome proliferation activators and functions as a serum hormone that regulates glucose homeostasis, lipid metabolism, and insulin sensitivity. This protein can also act as an apoptosis survival factor for vascular endothelial cells and can prevent metastasis by inhibiting vascular growth and tumor cell invasion. The C-terminal domain may be proteolytically-cleaved from the full-length secreted protein. Decreased expression of this gene has been associated with type 2 diabetes. Alternative splicing results in multiple transcript variants. This gene was previously referred to as ANGPTL2 but has been renamed ANGPTL4. [provided by RefSeq, Sep 2013] |
| Alternative Names | Angiopoietin like 4 antibody Angiopoietin related protein 4 antibody Angiopoietin-like protein 4 antibody Angiopoietin-related protein 4 antibody ANGL4_HUMAN antibody ANGPT L2 antibody ANGPT L4 antibody ANGPTL2 antibody Angptl4 antibody ARP4 antibody Angiopoietin like 4 antibody Angiopoietin related protein 4 antibody Angiopoietin-like protein 4 antibody Angiopoietin-related protein 4 antibody ANGL4_HUMAN antibody ANGPT L2 antibody ANGPT L4 antibody ANGPTL2 antibody Angptl4 antibody ARP4 antibody Fasting induced adipose factor antibody FIAF antibody HARP antibody Hepatic angiopoietin related protein antibody Hepatic fibrinogen/angiopoietin related protein antibody Hepatic fibrinogen/angiopoietin-related protein antibody HFARP antibody NL2 antibody Peroxisome proliferator-activated receptor (PPAR) gamma induced angiopoietin related protein antibody PGAR antibody pp1158 antibody PPARG angiopoietin related protein antibody PSEC0166 antibody TGQTL antibody UNQ171 antibody Weakly similar to angiopoietin 1 [H.sapiens] antibody |
| Molecular Weight(MW) | 45 kDa(Observed band size:60 kDa) |
| Cellular Localization | Secreted, extracellular space, extracellular matrix. |
WB
Western blot analysis of ANGPTL4 on different lysates with Rabbit anti-ANGPTL4 antibody at 1/2,000 dilution. Lysates/proteins at 20 µg/Lane. Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.| Application Notes | WB:1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
