WB
Western blot analysis of FLI1 on different lysates with Rabbit anti-FLI1 antibody at 1/1,000 dilution. Lysates/proteins at 20 µg/Lane. Exposure time: 2 minutes 6 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded human B cell lymphoma tissue with Rabbit anti-FLI1 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Product Name | FLI1 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide within human FLI1 aa 351-400 / 452. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:1000 IHC:1:1000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | FLI1 |
|---|---|
| Gene Synonyms | EWSR2 FLI-1 SIC-1 BDPLT21 |
| Gene Full Name | Fli-1 proto-oncogene, ETS transcription factor |
| Gene Summary | This gene encodes a transcription factor containing an ETS DNA-binding domain. The gene can undergo a t(11;22)(q24;q12) translocation with the Ewing sarcoma gene on chromosome 22, which results in a fusion gene that is present in the majority of Ewing sarcoma cases. An acute lymphoblastic leukemia-associated t(4;11)(q21;q23) translocation involving this gene has also been identified. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012] |
| Alternative Names | ERGB transcription factor antibody Ewing Sarcoma breakpoint region 2 antibody EWSR 2 antibody EWSR2 antibody FLI 1 antibody FLI 1 proto oncogene antibody Fli-1 proto-oncogene ETS transcription factor antibody FLI1 antibody FLI1 EWS fusion gene antibody FLI1 proto oncogene antibody ERGB transcription factor antibody Ewing Sarcoma breakpoint region 2 antibody EWSR 2 antibody EWSR2 antibody FLI 1 antibody FLI 1 proto oncogene antibody Fli-1 proto-oncogene ETS transcription factor antibody FLI1 antibody FLI1 EWS fusion gene antibody FLI1 proto oncogene antibody FLI1_HUMAN antibody Friend leukemia integration 1 (FLI1) transcription factor antibody Friend leukemia integration 1 transcription factor antibody Friend leukemia virus integration 1 antibody Proto-oncogene Fli-1 antibody Sarcoma breakpoint region 2 (EWSR2) antibody SIC 1 antibody SIC1 antibody Transcription factor ERGB antibody Viral integration region FLI1 antibody Viral integration region FLI1 mouse homolog of antibody |
| Molecular Weight(MW) | 51 kDa |
| Cellular Localization | Nucleus. |
WB
Western blot analysis of FLI1 on different lysates with Rabbit anti-FLI1 antibody at 1/1,000 dilution. Lysates/proteins at 20 µg/Lane. Exposure time: 2 minutes 6 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human B cell lymphoma tissue with Rabbit anti-FLI1 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Application Notes | WB:1:1000 IHC:1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
