WB
Western blot analysis of VE-Cadherin/CDH5 using anti-VE-Cadherin/CDH5 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-VE-Cadherin/CDH5 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.IHC
IHC analysis of VE-Cadherin/CDH5 using anti-VE-Cadherin/CDH5 antibody. VE-Cadherin/CDH5 was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. The tissue section was incubated with rabbit anti-VE-Cadherin/CDH5 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IF-P
IF analysis of VE-Cadherin/CDH5 using anti-VE-Cadherin/CDH5 antibody. VE-Cadherin/CDH5 was detected in a paraffin-embedded section of human lung cancer tissue. The tissue section was incubated with rabbit anti-VE-Cadherin/CDH5 Antibody at a dilution of 1:100. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red) was used as secondary antibody. The section was counterstained with DAPI (Blue).FC
Flow Cytometry analysis of HepG2 cells using anti-VE-Cadherin/CDH5 antibody. Overlay histogram showing HepG2 cells stained with Rabbit polyclonal antibody to VE-Cadherin/CDH5(Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-VE-Cadherin/CDH5 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample (Red line) was also used as a control.| Product Name | Rabbit polyclonal antibody to VE-Cadherin/CDH5 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E. coli-derived human VE Cadherin recombinant protein (Position: D48-R272). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCIF-PIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:400 IF-P:1:50-1:400 FC:1:50-1:200 |
|
| Species Reactivity | Human |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CDH5 |
|---|---|
| Gene Synonyms | 7B4 CD144 |
| Gene Full Name | cadherin 5 |
| Gene Summary | This gene encodes a classical cadherin of the cadherin superfamily. The encoded preproprotein is proteolytically processed to generate the mature glycoprotein. This calcium-dependent cell-cell adhesion molecule is comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Functioning as a classical cadherin by imparting to cells the ability to adhere in a homophilic manner, this protein plays a role in endothelial adherens junction assembly and maintenance. This gene is located in a gene cluster in a region on the long arm of chromosome 16 that is involved in loss of heterozygosity events in breast and prostate cancer. [provided by RefSeq, Nov 2015] |
| Alternative Names | 7B4 7B4 antigen Cadherin 5 CD144 CDH5 FLJ17376 Vascular endothelial cadherin VE cadherin |
| Molecular Weight(MW) | 88 kDa(Observed band size:88,120 kDa) |
| Cellular Localization | Cell junction,Cell membrane,Cytoplasm,Membrane. |
WB
Western blot analysis of VE-Cadherin/CDH5 using anti-VE-Cadherin/CDH5 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-VE-Cadherin/CDH5 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
IHC
IHC analysis of VE-Cadherin/CDH5 using anti-VE-Cadherin/CDH5 antibody. VE-Cadherin/CDH5 was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. The tissue section was incubated with rabbit anti-VE-Cadherin/CDH5 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IF-P
IF analysis of VE-Cadherin/CDH5 using anti-VE-Cadherin/CDH5 antibody. VE-Cadherin/CDH5 was detected in a paraffin-embedded section of human lung cancer tissue. The tissue section was incubated with rabbit anti-VE-Cadherin/CDH5 Antibody at a dilution of 1:100. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red) was used as secondary antibody. The section was counterstained with DAPI (Blue).
FC
Flow Cytometry analysis of HepG2 cells using anti-VE-Cadherin/CDH5 antibody. Overlay histogram showing HepG2 cells stained with Rabbit polyclonal antibody to VE-Cadherin/CDH5(Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-VE-Cadherin/CDH5 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample (Red line) was also used as a control.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 IF-P:1:50-1:400 FC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt，-20℃ as supplied. |
| Storage Buffer | 500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
