WB
Positive control:Human liver tissue lysate. Recommended dilution: 500-2000 . Gel: 8%SDS-PAGE . Lysate: 40 μg . Lane: Human liver tissue lysate . Primary antibody:SIGLEC8 Antibody at dilution 1/500 . Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution . Exposure time: 2 minutes.IHC
Predicted cell location: Cytoplasm . Positive control: Human prostate cancer . Recommended dilution: 50-200. The image on the left is immunohistochemistry of paraffin-embedded Human prostate cancer tissue using SIGLEC8 Antibody at dilution 1/75, on the right is treated with synthetic peptide. (Original magnification: ×200) .| Product Name | Anti-SIGLEC8 rabbit polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide of human SIGLEC8. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:500-1:2000 IHC:1:50-1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SIGLEC8 |
|---|---|
| Gene Synonyms | SAF2 SIGLEC-8 SIGLEC8L |
| Gene Full Name | sialic acid binding Ig like lectin 8 |
| Gene Summary | Sialic acid-binding immunoglobulin (Ig)-like lectins, or SIGLECs (e.g., CD33 (MIM 159590)), are a family of type 1 transmembrane proteins each having a unique expression pattern, mostly in hemopoietic cells. SIGLEC8 is a member of the CD33-like subgroup of SIGLECs, which are localized to 19q13.3-q13.4 and have 2 conserved cytoplasmic tyrosine-based motifs: an immunoreceptor tyrosine-based inhibitory motif, or ITIM (see MIM 604964), and a motif homologous to one identified in signaling lymphocyte activation molecule (SLAM; MIM 603492) that mediates an association with SLAM-associated protein (SAP; MIM 300490) (summarized by Foussias et al., 2000 [PubMed 11095983]).[supplied by OMIM, May 2010] |
| Alternative Names | SAF2 SIGLEC-8 SIGLEC8L |
| Molecular Weight(MW) | 54 kDa |
| Cellular Localization | Membrane. |
WB
Positive control:Human liver tissue lysate. Recommended dilution: 500-2000 . Gel: 8%SDS-PAGE . Lysate: 40 μg . Lane: Human liver tissue lysate . Primary antibody:SIGLEC8 Antibody at dilution 1/500 . Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution . Exposure time: 2 minutes.
IHC
Predicted cell location: Cytoplasm . Positive control: Human prostate cancer . Recommended dilution: 50-200. The image on the left is immunohistochemistry of paraffin-embedded Human prostate cancer tissue using SIGLEC8 Antibody at dilution 1/75, on the right is treated with synthetic peptide. (Original magnification: ×200) .| Application Notes | WB:1:500-1:2000 IHC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -20°C. |
| Storage Buffer | pH7.4 PBS, 0.05% NaN3, 40% Glycerol. |
