Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.Application
Antibody is used in Western blot to detect Siglec8 in mouse liver tissue lysate (lnae 1) and in HL60 cell lysate (lane 2).| Product Name | SIGLEC8 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | SIGLEC8 is a putative adhesion molecule that mediates sialic-acid dependent binding to cells. It preferentially binds to alpha2,3-linked sialic acid. and also binds to alpha2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. SIGLEC8 is expressed specifically on eosinophils. The protein contains 1 copy of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in downmodulation of cellular responses. The phosphorylated ITIM motif binds to the SH2 domain of PTPN6/SHP-1. The SIGLEC8 gene belongs to the immunoglobulin superfamily.1) Foussias, G., et al., Biochem. Biophys. Res. Commun. 278(3):775-781 (2000). |
| Immunogen | This SIGLEC8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 373-406 amino acids from the C-terminal region of human SIGLEC8. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Gene Symbol | SIGLEC8 |
|---|---|
| Alternative Names | Sialic acid-binding Ig-like lectin 8 Siglec-8 Sialoadhesin family member 2 SAF-2 SIGLEC8 SAF2 |
| Molecular Weight(MW) | 54 kDa |
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.
Application
Antibody is used in Western blot to detect Siglec8 in mouse liver tissue lysate (lnae 1) and in HL60 cell lysate (lane 2).| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
