WB
Western blot analysis of ARID1A using anti-ARID1A antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ARID1A antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.IHC
IHC analysis of ARID1A using anti-ARID1A antibody. ARID1A was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. The tissue section was incubated with rabbit anti-ARID1A Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
IHC analysis of ARID1A using anti-ARID1A antibody . ARID1A was detected in a paraffin-embedded section of human spleen tissue. The tissue section was incubated with rabbit anti-ARID1A Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
IHC analysis of ARID1A using anti-ARID1A antibody ARID1A was detected in a paraffin-embedded section of rat thymus tissue. The tissue section was incubated with rabbit anti-ARID1A Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.ICC/IF
ICC/IF analysis of ARID1A using anti-ARID1A antibody and anti-Beta Tubulin antibody. ARID1A was detected in an immunocytochemical section of A431 cells. The section was incubated with rabbit anti-ARID1A Antibody at a dilution of 1:100. Fluoro488-conjugated Anti-rabbit IgG Secondary Antibody (green) and Cy3-conjugated Anti-mouse IgG Secondary Antibody (red) were used as secondary antibody.FC
Flow Cytometry analysis of K562 cells using anti-ARID1A antibody. Overlay histogram showing K562 cells stained with anti-ARID1A antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ARID1A Antibody (1:100). Fluoro488 conjugated goat anti-rabbit IgG (1:100) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.| Product Name | Rabbit polyclonal antibody to ARID1A |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human ARID1A recombinant protein (Position: R857-Q1552). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 FC:1:50-1:200 |
|
| Species Reactivity | HumanRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ARID1A |
|---|---|
| Gene Synonyms | ELD B120 CSS2 OSA1 P270 hELD BM029 MRD14 hOSA1 BAF250 C1orf4 BAF250a SMARCF1 |
| Gene Full Name | AT-rich interaction domain 1A |
| Gene Summary | This gene encodes a member of the SWI/SNF family, whose members have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. It possesses at least two conserved domains that could be important for its function. First, it has a DNA-binding domain that can specifically bind an AT-rich DNA sequence known to be recognized by a SNF/SWI complex at the beta-globin locus. Second, the C-terminus of the protein can stimulate glucocorticoid receptor-dependent transcriptional activation. It is thought that the protein encoded by this gene confers specificity to the SNF/SWI complex and may recruit the complex to its targets through either protein-DNA or protein-protein interactions. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| Alternative Names | ARID1A B120 BAF250 BAF250a BM029 BRG1 associated factor 250 BRG1 associated factor 250a C1orf4 hELD hOSA1 Osa homolog 1 OSA1 P270 SMARCF1 SWI like protein SWI/SNF complex protein p270 |
| Molecular Weight(MW) | 242 kDa(Observed band size: 250-270 kDa) |
| Cellular Localization | Nucleus |
WB
Western blot analysis of ARID1A using anti-ARID1A antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ARID1A antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
IHC
IHC analysis of ARID1A using anti-ARID1A antibody. ARID1A was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. The tissue section was incubated with rabbit anti-ARID1A Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of ARID1A using anti-ARID1A antibody . ARID1A was detected in a paraffin-embedded section of human spleen tissue. The tissue section was incubated with rabbit anti-ARID1A Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of ARID1A using anti-ARID1A antibody ARID1A was detected in a paraffin-embedded section of rat thymus tissue. The tissue section was incubated with rabbit anti-ARID1A Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
ICC/IF
ICC/IF analysis of ARID1A using anti-ARID1A antibody and anti-Beta Tubulin antibody. ARID1A was detected in an immunocytochemical section of A431 cells. The section was incubated with rabbit anti-ARID1A Antibody at a dilution of 1:100. Fluoro488-conjugated Anti-rabbit IgG Secondary Antibody (green) and Cy3-conjugated Anti-mouse IgG Secondary Antibody (red) were used as secondary antibody.
FC
Flow Cytometry analysis of K562 cells using anti-ARID1A antibody. Overlay histogram showing K562 cells stained with anti-ARID1A antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ARID1A Antibody (1:100). Fluoro488 conjugated goat anti-rabbit IgG (1:100) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 FC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| Storage Buffer | 500ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
