Application
Immunofluorescence of ARID1A in mouse brain tissue with ARID1A antibody at 20 μg/ml.Application
Immunofluorescence of ARID1A in mouse brain tissue with ARID1A antibody at 20 μg/ml.Application
Immunohistochemistry of ARID1A in mouse brain tissue with ARID1A antibody at 5 μg/mL.Application
Western blot analysis of ARID1A in SK-N-SH cell lysate with ARID1A antibody at (A) 1 and (B) 2 μg/ml.| Product Name | ARID1A Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AT rich interactive domain 1A (SWI-like), RP1-50O24.1, B120, BAF250, BAF250a, BM029, C1orf4, ELD, hELD, MRD14, OSA1, hOSA1, P270, SMARCF1 |
| Product description | The ARID1A protein is a member of the SWI/SNF family, whose members are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. ARID1A is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin (1). It possesses a DNA-binding domain that can specifically bind an AT-rich DNA sequence known to be recognized by a SNF/SWI complex at the beta-globin locus. The C-terminus of the protein can stimulate glucocorticoid receptor-dependent transcriptional activation. It is thought that ARID1A confers specificity to the SNF/SWI complex and may recruit the complex to its targets through either protein-DNA or protein-protein interactions (2).1) Martens JA and Winston F. Recent advances in understanding chromatin remodeling by Swi/Snf complexes. Curr. Opin. Genet. Dev. 2003; 13:136-42. |
| Immunogen | ARID1A antibody was raised against an 18 amino acid peptide near the amino terminus of human ARID1A. The immunogen is located within amino acids 580 - 630 of ARID1A. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| ARID1A antibody can be used for detection of ARID1A by Western blot at 1 - 2 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ARID1A |
|---|---|
| Alternative Names | AT rich interactive domain 1A (SWI-like) RP1-50O24.1 B120 BAF250 BAF250a BM029 C1orf4 ELD hELD MRD14 OSA1 hOSA1 P270 SMARCF1 |
| Molecular Weight(MW) | Predicted: 133, 227, 251 kDa Observed: 130, 260 kDa |
| Tissue Specificity | ARID1A antibody is human, mouse and rat reactive. Multiple isoforms of ARID1A protein are known to exist. |
Application
Immunofluorescence of ARID1A in mouse brain tissue with ARID1A antibody at 20 μg/ml.
Application
Immunofluorescence of ARID1A in mouse brain tissue with ARID1A antibody at 20 μg/ml.
Application
Immunohistochemistry of ARID1A in mouse brain tissue with ARID1A antibody at 5 μg/mL.
Application
Western blot analysis of ARID1A in SK-N-SH cell lysate with ARID1A antibody at (A) 1 and (B) 2 μg/ml.| Positive Control | 1) Cat. No. 1220 - SK-N-SH Cell Lysate |
|---|---|
| Application Notes | ARID1A antibody can be used for detection of ARID1A by Western blot at 1 - 2 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | ARID1A antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | ARID1A antibody is supplied in PBS containing 0.02% sodium azide. |
