WB
Western blot analysis of PKM2/PKM using anti-PKM2/PKM antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-PKM2/PKM antigen affinity purified monoclonal antibody at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.IHC
IHC analysis of PKM2/PKM using anti-PKM2/PKM antibody. PKM2/PKM was detected in a paraffin-embedded section of human liver cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-PKM2/PKM Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.IHC
IHC analysis of PKM2/PKM using anti-PKM2/PKM antibody. PKM2/PKM was detected in a paraffin-embedded section of Differentiated adenocarcinoma of the human rectum tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-PKM2/PKM Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.ICC/IF
ICC/IF analysis of PKM2/PKM using anti-PKM2/PKM antibody. PKM2/PKM was detected in an immunocytochemical section of A549 cells. The section was incubated with mouse anti-PKM2/PKM Antibody at a dilution of 1:100. Fluoro488-conjugated Anti-mouse IgG Secondary Antibody (green) was used as secondary antibody. The section was counterstained with DAPI (Blue).FC
Flow Cytometry analysis of Hela cells using anti-PKM2/PKM antibody. Overlay histogram showing Hela cells stained with anti-PKM2/PKM antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-PKM2/PKM Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-mouse IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Product Name | Mouse monoclonal antibody to PKM2/PKM |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human PKM2, different from the related mouse sequence by five amino acids, and from the related rat sequence by four amino acids. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2b |
| Host Species | Mouse |
| Tested Applications | FCICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 FC:1:50-1:200 |
|
| Species Reactivity | Human |
| Concentration | 0.5mg/ml |
| Purification | Protein G |
| Gene Symbol | PKM |
|---|---|
| Gene Synonyms | PK3 TCB p58 OIP3 PKM2 CTHBP THBP1 HEL-S-30 |
| Gene Full Name | pyruvate kinase M1/2 |
| Gene Summary | This gene encodes a protein involved in glycolysis. The encoded protein is a pyruvate kinase that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate to ADP, generating ATP and pyruvate. This protein has been shown to interact with thyroid hormone and may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Several alternatively spliced transcript variants encoding a few distinct isoforms have been reported. [provided by RefSeq, May 2011] |
| Alternative Names | CTHBP OIP 3 OIP3 Opa interacting protein 3 p58 PK2 PK3 PKM PKM2 PK-M2 PKM2 pyruvate kinase muscle Pyruvate kinase 2/3 Pyruvate kinase isozymes M1/M2 Pyruvate kinase muscle isozyme TCB THBP1 Tumor M2 PK |
| Molecular Weight(MW) | 58 kDa |
| Cellular Localization | Cytoplasm,Nucleus |
WB
Western blot analysis of PKM2/PKM using anti-PKM2/PKM antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-PKM2/PKM antigen affinity purified monoclonal antibody at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
IHC
IHC analysis of PKM2/PKM using anti-PKM2/PKM antibody. PKM2/PKM was detected in a paraffin-embedded section of human liver cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-PKM2/PKM Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IHC
IHC analysis of PKM2/PKM using anti-PKM2/PKM antibody. PKM2/PKM was detected in a paraffin-embedded section of Differentiated adenocarcinoma of the human rectum tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-PKM2/PKM Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
ICC/IF
ICC/IF analysis of PKM2/PKM using anti-PKM2/PKM antibody. PKM2/PKM was detected in an immunocytochemical section of A549 cells. The section was incubated with mouse anti-PKM2/PKM Antibody at a dilution of 1:100. Fluoro488-conjugated Anti-mouse IgG Secondary Antibody (green) was used as secondary antibody. The section was counterstained with DAPI (Blue).
FC
Flow Cytometry analysis of Hela cells using anti-PKM2/PKM antibody. Overlay histogram showing Hela cells stained with anti-PKM2/PKM antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-PKM2/PKM Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-mouse IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 FC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| Storage Buffer | 500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
