WB
Western blot analysis of HHAT using anti-HHAT antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U2OS whole cell lysates, Lane 2: human SIHA whole cell lysates, Lane 3: rat ovary tissue lysates, Lane 4: rat testis tissue lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-HHAT antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.IHC
HHAT was detected in a paraffin-embedded section of human testis tissue. The tissue section was incubated with rabbit anti-HHAT Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.ICC/IF
HHAT was detected in an immunocytochemical section of U2OS cells. The section was incubated with rabbit anti-HHAT Antibody at a dilution of 1:100. Cy3-Conjugated Anti-rabbit IgG Secondary Antibody (Red) and Fluoro488-conjugated Anti-mouse IgG Secondary Antibody (Green) were used as secondary antibody. The section was counterstained with DAPI (Blue).| Product Name | Rabbit polyclonal antibody to HHAT |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human HHAT recombinant protein (Position: R30-G247). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 |
|
| Species Reactivity | HumanRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | HHAT |
|---|---|
| Gene Synonyms | Skn NNMS SKI1 MART2 |
| Gene Full Name | hedgehog acyltransferase |
| Gene Summary | 'Skinny hedgehog' (SKI1) encodes an enzyme that acts within the secretory pathway to catalyze amino-terminal palmitoylation of 'hedgehog' (see MIM 600725).[supplied by OMIM, Jul 2002] |
| Alternative Names | HHAT MART2 SKI1 Protein-cysteine N-palmitoyltransferase HHAT EC 2.3.1.- Hedgehog acyltransferase Melanoma antigen recognized by T-cells 2 MART-2 Skinny hedgehog protein 1 |
| Molecular Weight(MW) | 57 kDa |
| Cellular Localization | Endoplasmic reticulum membrane,Golgi apparatus membrane. |
WB
Western blot analysis of HHAT using anti-HHAT antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U2OS whole cell lysates, Lane 2: human SIHA whole cell lysates, Lane 3: rat ovary tissue lysates, Lane 4: rat testis tissue lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-HHAT antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
IHC
HHAT was detected in a paraffin-embedded section of human testis tissue. The tissue section was incubated with rabbit anti-HHAT Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
ICC/IF
HHAT was detected in an immunocytochemical section of U2OS cells. The section was incubated with rabbit anti-HHAT Antibody at a dilution of 1:100. Cy3-Conjugated Anti-rabbit IgG Secondary Antibody (Red) and Fluoro488-conjugated Anti-mouse IgG Secondary Antibody (Green) were used as secondary antibody. The section was counterstained with DAPI (Blue).| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| Storage Buffer | 500ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
