WB
Western blot analysis of FCER1G using anti-FCER1G antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-FCER1G antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.IHC
IHC analysis of FCER1G using anti-FCER1G antibody. FCER1G was detected in a paraffin-embedded section of human spleen tissue. The tissue section was incubated with rabbit anti-FCER1G Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
IHC analysis of FCER1G using anti-FCER1G antibody. FCER1G was detected in a paraffin-embedded section of mouse spleen tissue. The tissue section was incubated with rabbit anti-FCER1G Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
IHC analysis of FCER1G using anti-FCER1G antibody. FCER1G was detected in a paraffin-embedded section of rat spleen tissue. The tissue section was incubated with rabbit anti-FCER1G Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IP
IP analysis of FCER1G using anti-FCER1G antibody in HEL whole cell lysate. Western blot analysis of FCER1G using anti- FCER1G antibody. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti- FCER1G antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.| Product Name | Rabbit polyclonal antibody to FCER1G |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human FCER1G recombinant protein (Position: Y43-Q86). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCIPWB |
| WB:1:500-1:2000 IHC:1:50-1:400 IP:1:250-1:300 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | FCER1G |
|---|---|
| Gene Synonyms | FCRG |
| Gene Full Name | Fc epsilon receptor Ig |
| Gene Summary | The high affinity IgE receptor is a key molecule involved in allergic reactions. It is a tetramer composed of 1 alpha, 1 beta, and 2 gamma chains. The gamma chains are also subunits of other Fc receptors. [provided by RefSeq, Jul 2008] |
| Alternative Names | FcRgamma FCRG FceRI gamma Fc-epsilon RI-gamma Fc receptor gamma-chain |
| Molecular Weight(MW) | 10 kDa(Observed band size:12 kDa) |
| Cellular Localization | Cell membrane,Membrane. |
WB
Western blot analysis of FCER1G using anti-FCER1G antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-FCER1G antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
IHC
IHC analysis of FCER1G using anti-FCER1G antibody. FCER1G was detected in a paraffin-embedded section of human spleen tissue. The tissue section was incubated with rabbit anti-FCER1G Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of FCER1G using anti-FCER1G antibody. FCER1G was detected in a paraffin-embedded section of mouse spleen tissue. The tissue section was incubated with rabbit anti-FCER1G Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of FCER1G using anti-FCER1G antibody. FCER1G was detected in a paraffin-embedded section of rat spleen tissue. The tissue section was incubated with rabbit anti-FCER1G Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IP
IP analysis of FCER1G using anti-FCER1G antibody in HEL whole cell lysate. Western blot analysis of FCER1G using anti- FCER1G antibody. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti- FCER1G antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 IP:1:250-1:300 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| Storage Buffer | 500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
