WB
Western blot analysis of SAA1 + SAA2 on different lysates with Rabbit anti-SAA1 + SAA2 antibody at 1/5,000 dilution. Lysates/proteins at 20 µg/Lane. Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IF-F
Application: IHC-Fr. Species: Mouse. Site: liver. Sample: Frozen section. Antibody concentration: 1/1,000. Antigen retrieval: Not required.| Product Name | SAA1 + SAA2 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within mouse SAA1 aa 1-122. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IF-FWB |
| WB:1:5000 IF-F:1:1000 |
|
| Species Reactivity | Mouse |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | SAA1 |
|---|---|
| Gene Synonyms | SAA PIG4 TP53I4 |
| Gene Full Name | serum amyloid A1 |
| Gene Summary | This gene encodes a member of the serum amyloid A family of apolipoproteins. The encoded preproprotein is proteolytically processed to generate the mature protein. This protein is a major acute phase protein that is highly expressed in response to inflammation and tissue injury. This protein also plays an important role in HDL metabolism and cholesterol homeostasis. High levels of this protein are associated with chronic inflammatory diseases including atherosclerosis, rheumatoid arthritis, Alzheimer's disease and Crohn's disease. This protein may also be a potential biomarker for certain tumors. Finally, antimicrobial activity against S. aureus and E. coli resides in the N-terminal portion of the mature protein. Alternate splicing results in multiple transcript variants that encode the same protein. A pseudogene of this gene is found on chromosome 11. [provided by RefSeq, Jul 2020] |
| Alternative Names | amyloid A serum antibody Amyloid fibril protein AA antibody Amyloid protein A antibody MGC111216 antibody PIG4 antibody SAA antibody Serum amyloid A protein antibody serum amyloid A-1 protein antibody serum amyloid A1 antibody TP53I4 antibody Tumor protein p53 inducible protein 4 antibody Serum amyloid A-2 protein Amyloid protein A Saa2 |
| Molecular Weight(MW) | 14 kDa(Observed band size:12 kDa) |
| Cellular Localization | Secreted. |
WB
Western blot analysis of SAA1 + SAA2 on different lysates with Rabbit anti-SAA1 + SAA2 antibody at 1/5,000 dilution. Lysates/proteins at 20 µg/Lane. Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IF-F
Application: IHC-Fr. Species: Mouse. Site: liver. Sample: Frozen section. Antibody concentration: 1/1,000. Antigen retrieval: Not required.| Application Notes | WB:1:5000 IF-F:1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage Buffer | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
