WB
Western blot analysis of HSPB8 using anti-HSPB8 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-HSPB8 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.IHC
IHC analysis of HSPB8 using anti-HSPB8 antibody. HSPB8 was detected in a paraffin-embedded section of mouse pancreas tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-HSPB8 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.IHC
IHC analysis of HSPB8 using anti-HSPB8 antibody. HSPB8 was detected in a paraffin-embedded section of rat pancreas tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-HSPB8 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.IHC
IHC analysis of HSPB8 using anti-HSPB8 antibody. HSPB8 was detected in a paraffin-embedded section of human lung cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-HSPB8 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.ICC/IF
ICC/IF analysis of HSPB8 using anti-HSPB8 antibody. HSPB8 was detected in an immunocytochemical section of MCF-7 cells. The section was incubated with rabbit anti-HSPB8 Antibody at a dilution of 1:100. Fluoro594-conjugated Anti-rabbit IgG Secondary Antibody (red) was used as secondary antibody. The section was counterstained with DAPI (Blue).IP
IP analysis of HSPB8 using anti-HSPB8 antibody in MCF-7 whole cell lysate. Western blot analysis of HSPB8 using anti- HSPB8 antibody. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti- HSPB8 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.FC
Flow Cytometry analysis of U2OS cells using anti-HSPB8 antibody. Overlay histogram showing U2OS cells stained with Rabbit polyclonal antibody to HSPB8 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HSPB8 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Product Name | Rabbit polyclonal antibody to HSPB8 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human HSPB8/Hsp22 recombinant protein (Position: M1-T196). Human HSPB8/Hsp22 shares 94.4% and 95.4% amino acid (aa) sequence identity with mouse and rat HSPB8/Hsp22, respectively. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIHCIPWB |
| WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 IP:1:250-1:300 FC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | HSPB8 |
|---|---|
| Gene Synonyms | H11 HMN2 CMT2L DHMN2 E2IG1 HMN2A HMND2 HSP22 MFM13 HSPB8-N1 HSPB8-N2 |
| Gene Full Name | heat shock protein family B (small) member 8 |
| Gene Summary | The protein encoded by this gene belongs to the superfamily of small heat-shock proteins containing a conservative alpha-crystallin domain at the C-terminal part of the molecule. The expression of this gene in induced by estrogen in estrogen receptor-positive breast cancer cells, and this protein also functions as a chaperone in association with Bag3, a stimulator of macroautophagy. Thus, this gene appears to be involved in regulation of cell proliferation, apoptosis, and carcinogenesis, and mutations in this gene have been associated with different neuromuscular diseases, including Charcot-Marie-Tooth disease. [provided by RefSeq, Jul 2008] |
| Alternative Names | Alpha crystallin C chain CMT2L CRYAC DHMN2 E2 induced gene 1 protein E2IG1 H11 heat shock 22kDa protein 8 Heat shock protein beta 8 HMN2 HMN2A HSP22 HSPB8 Protein kinase H11 |
| Molecular Weight(MW) | 22 kDa |
| Cellular Localization | Cytoplasm,Nucleus |
WB
Western blot analysis of HSPB8 using anti-HSPB8 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-HSPB8 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
IHC
IHC analysis of HSPB8 using anti-HSPB8 antibody. HSPB8 was detected in a paraffin-embedded section of mouse pancreas tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-HSPB8 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IHC
IHC analysis of HSPB8 using anti-HSPB8 antibody. HSPB8 was detected in a paraffin-embedded section of rat pancreas tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-HSPB8 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IHC
IHC analysis of HSPB8 using anti-HSPB8 antibody. HSPB8 was detected in a paraffin-embedded section of human lung cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-HSPB8 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
ICC/IF
ICC/IF analysis of HSPB8 using anti-HSPB8 antibody. HSPB8 was detected in an immunocytochemical section of MCF-7 cells. The section was incubated with rabbit anti-HSPB8 Antibody at a dilution of 1:100. Fluoro594-conjugated Anti-rabbit IgG Secondary Antibody (red) was used as secondary antibody. The section was counterstained with DAPI (Blue).
IP
IP analysis of HSPB8 using anti-HSPB8 antibody in MCF-7 whole cell lysate. Western blot analysis of HSPB8 using anti- HSPB8 antibody. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti- HSPB8 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
FC
Flow Cytometry analysis of U2OS cells using anti-HSPB8 antibody. Overlay histogram showing U2OS cells stained with Rabbit polyclonal antibody to HSPB8 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HSPB8 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 IP:1:250-1:300 FC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt，-20℃ as supplied. |
| Storage Buffer | 500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
