WB
Western blot analysis of RCC1 using anti-RCC1 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-RCC1 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.IHC
IHC analysis of RCC1 using anti-RCC1 antibody. RCC1 was detected in a paraffin-embedded section of human rectal cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-RCC1 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.ICC/IF
ICC/IF analysis of RCC1 using anti-RCC1 antibody. RCC1 was detected in an immunocytochemical section of Hela cells. The section was incubated with rabbit anti-RCC1 Antibody at a dilution of 1:100. Fluoro594-conjugated Anti-rabbit IgG Secondary Antibody (red) was used as secondary antibody. The section was counterstained with DAPI (Blue).IF-F
IF analysis using anti-RCC1 antibody. detected in paraffin-embedded section of human intestine cancer tissue. The tissue section were stained using the Fluoro488 conjugated Anti-rabbit IgG Secondary Antibody (green) and counterstained with DAPI (blue).FC
Flow Cytometry analysis of K562 cells using anti-RCC1 antibody. Overlay histogram showing K562 cells stained with Rabbit polyclonal antibody to RCC1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RCC1 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Product Name | Rabbit polyclonal antibody to RCC1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human RCC1 recombinant protein (Position: A14-S421). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIF-PIHCWB |
| WB:1:1000-1:5000 IHC:1:50-1:400 ICC/IF:1:50-1:400 IF-F:1:50-1:400 FC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RCC1 |
|---|---|
| Gene Synonyms | CHC1 IIAAN RCC1-I |
| Gene Full Name | regulator of chromosome condensation 1 |
| Gene Summary | Enables several functions, including guanyl-nucleotide exchange factor activity; nucleosomal DNA binding activity; and protein heterodimerization activity. Involved in G1/S transition of mitotic cell cycle; regulation of mitotic nuclear division; and spindle organization. Located in chromatin; cytoplasm; and nuclear lumen. Part of protein-containing complex. [provided by Alliance of Genome Resources, Apr 2025] |
| Alternative Names | Cell cycle regulatory protein CHC1 RCC1 RCC1 I |
| Molecular Weight(MW) | 45 kDa |
| Cellular Localization | Chromosome,Cytoplasm,Nucleus. |
WB
Western blot analysis of RCC1 using anti-RCC1 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-RCC1 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
IHC
IHC analysis of RCC1 using anti-RCC1 antibody. RCC1 was detected in a paraffin-embedded section of human rectal cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-RCC1 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
ICC/IF
ICC/IF analysis of RCC1 using anti-RCC1 antibody. RCC1 was detected in an immunocytochemical section of Hela cells. The section was incubated with rabbit anti-RCC1 Antibody at a dilution of 1:100. Fluoro594-conjugated Anti-rabbit IgG Secondary Antibody (red) was used as secondary antibody. The section was counterstained with DAPI (Blue).
IF-F
IF analysis using anti-RCC1 antibody. detected in paraffin-embedded section of human intestine cancer tissue. The tissue section were stained using the Fluoro488 conjugated Anti-rabbit IgG Secondary Antibody (green) and counterstained with DAPI (blue).
FC
Flow Cytometry analysis of K562 cells using anti-RCC1 antibody. Overlay histogram showing K562 cells stained with Rabbit polyclonal antibody to RCC1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RCC1 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Application Notes | WB:1:1000-1:5000 IHC:1:50-1:400 ICC/IF:1:50-1:400 IF-F:1:50-1:400 FC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt，-20℃ as supplied. |
| Storage Buffer | 500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
