Application
Western blot analysis of RCC1 Antibody in 293, MDA-MB231 cell line lysates (35ug/lane). RCC1 (arrow) was detected using the purified Pab.Application
RCC1 Antibody IHC analysis in formalin fixed and paraffin embedded mouse brain followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the RCC1 Antibody for immunohistochemistry. Clinical relevance has not been evaluated.Application
RCC1 Antibody flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | RCC1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RCC1; CHC1; Regulator of chromosome condensation; Cell cycle regulatory protein; Chromosome condensation protein 1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CHC1 |
|---|---|
| Alternative Names | RCC1 CHC1 Regulator of chromosome condensation Cell cycle regulatory protein Chromosome condensation protein 1 |
| Molecular Weight(MW) | 44969 Da |
| Function | Guanine-nucleotide releasing factor that promotes the exchange of Ran-bound GDP by GTP. Involved in the regulation of onset of chromosome condensation in the S phase. Binds both to the nucleosomes and double-stranded DNA. RCC1-Ran complex (together with other proteins) acts as a component of a signal transmission pathway that detects unreplicated DNA. Plays a key role in nucleo- cytoplasmic transport, mitosis and nuclear-envelope assembly |
| Tissue Specificity | This RCC1 antibody is generated from rabbits immunized with human RCC1 recombinant protein. |
| Cellular Localization | Nucleus. Cytoplasm. Note=Becomes dispersed throughout the cytoplasm during mitosis |
| Entrez Gene | 1104 |
|---|
Application
Western blot analysis of RCC1 Antibody in 293, MDA-MB231 cell line lysates (35ug/lane). RCC1 (arrow) was detected using the purified Pab.
Application
RCC1 Antibody IHC analysis in formalin fixed and paraffin embedded mouse brain followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the RCC1 Antibody for immunohistochemistry. Clinical relevance has not been evaluated.
Application
RCC1 Antibody flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
