WB
Western blot analysis of extracts from Rat liver tissue using LRMP Rabbit mAb at 1:2000.WB
Western blot analysis of extracts from Ramos cells using LRMP Rabbit mAb at 1:1000.ICC/IF
Immunofluorescence analysis of Jurkat cells labelling LRMP with LRMP Rabbit mAb. The cells were fixed with 4% PFA (10min, RT) followed by treatment with 0.1% Triton X-100 (10min, RT), and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with LRMP Rabbit mAb (1:100) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 (shown in green). Nuclear DNA was labeled in blue with DAPI. Control: Secondary antibody only.| Product Name | LRMP Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein of human LRMP. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| WB:1:1000-1:5000 ICC/IF:1:100-1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | IRAG2 |
|---|---|
| Gene Synonyms | JAW1 LRMP |
| Gene Full Name | inositol 1,4,5-triphosphate receptor associated 2 |
| Gene Summary | The protein encode dby this gene is expressed in a developmentally regulated manner in lymphoid cell lines and tissues. The protein is localized to the cytoplasmic face of the endoplasmic reticulum. [provided by RefSeq, Jul 2008] |
| Alternative Names | JAW1 |
| Molecular Weight(MW) | 62 kDa |
| Cellular Localization | Chromosome,Cytoplasm,Cytoskeleton,Endoplasmic reticulum,Membrane,Nucleus. |
WB
Western blot analysis of extracts from Rat liver tissue using LRMP Rabbit mAb at 1:2000.
WB
Western blot analysis of extracts from Ramos cells using LRMP Rabbit mAb at 1:1000.
ICC/IF
Immunofluorescence analysis of Jurkat cells labelling LRMP with LRMP Rabbit mAb. The cells were fixed with 4% PFA (10min, RT) followed by treatment with 0.1% Triton X-100 (10min, RT), and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with LRMP Rabbit mAb (1:100) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 (shown in green). Nuclear DNA was labeled in blue with DAPI. Control: Secondary antibody only.| Application Notes | WB:1:1000-1:5000 ICC/IF:1:100-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C.Stable for 12 months from date of receipt. |
| Storage Buffer | Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA |
