WB
Western blot analysis of REG4 expression in mouse pancreas (A) whole cell lysates.IHC
Immunohistochemical analysis of REG4 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.ICC/IF
Immunofluorescent analysis of REG4 staining in MDAMB231 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).| Product Name | Anti-REG4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the center region of human REG4. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500-1:1000 IHC:1:100-1:200 ICC/IF:1:50-1:200 |
|
| Species Reactivity | HumanMousePigRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | REG4 |
|---|---|
| Gene Synonyms | GISP RELP REG-IV |
| Gene Full Name | regenerating family member 4 |
| Gene Summary | Enables heparin binding activity and mannan binding activity. Predicted to act upstream of or within response to bacterium. Located in cytoplasm. [provided by Alliance of Genome Resources, Jul 2025] |
| Alternative Names | GISP RELP Regenerating islet-derived protein 4 REG-4 Gastrointestinal secretory protein REG-like protein Regenerating islet-derived protein IV Reg IV |
| Molecular Weight(MW) | 18 kDa |
| Cellular Localization | Secreted. |
WB
Western blot analysis of REG4 expression in mouse pancreas (A) whole cell lysates.
IHC
Immunohistochemical analysis of REG4 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
ICC/IF
Immunofluorescent analysis of REG4 staining in MDAMB231 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).| Application Notes | WB:1:500-1:1000 IHC:1:100-1:200 ICC/IF:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles. |
| Storage Buffer | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
