WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-eNOS (Phospho Ser1177) antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody.IHC
Human kidney was stained with anti-eNOS (Phospho Ser1177) Rabbit antibody.ICC/IF
Immunofluorescence analysis of A549. 1,primary Antibody (red) was diluted at 1:200 (4℃ overnight). 2, Goat Anti Rabbit IgG (H&L) - Alexa Fluor 594 Secondary antibody was diluted at 1:1000 (room temperature, 50min).3, DAPI (blue) 10min.ICC/IF
Immunofluorescence analysis of Hela cell. 1,NOS3 (phospho Ser1177) Antibody (green) was diluted at 1:200 (4℃ overnight). 2, Goat Anti Rabbit Alexa Fluor 488 was diluted at 1:1000 (room temperature, 50min). 3 DAPI (blue) 10min.| Product Name | eNOS (Phospho Ser1177) Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Modification | p-Ser1177 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG,K |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:2000-1:10000 IHC:1:200-1:1000 ICC/IF:1:200-1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NOS3 |
|---|---|
| Gene Synonyms | cNOS eNOS ECNOS MYMY8 EC-NOS NOSIII |
| Gene Full Name | nitric oxide synthase 3 |
| Gene Summary | Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Alternative splicing and the use of alternative promoters results in multiple transcript variants. [provided by RefSeq, Oct 2016] |
| Alternative Names | NOS3 Nitric oxide synthase endothelial Constitutive NOS cNOS EC-NOS Endothelial NOS eNOS NOS type III NOSIII |
| Molecular Weight(MW) | 133 kDa |
| Cellular Localization | Cell membrane. Membrane , caveola. Cytoplasm , cytoskeleton. Golgi apparatus. Specifically associates with actin cytoskeleton in the G2 phase of the cell cycle; which is favored by interaction with NOSIP and results in a reduced enzymatic activity. |
WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-eNOS (Phospho Ser1177) antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody.
IHC
Human kidney was stained with anti-eNOS (Phospho Ser1177) Rabbit antibody.
ICC/IF
Immunofluorescence analysis of A549. 1,primary Antibody (red) was diluted at 1:200 (4℃ overnight). 2, Goat Anti Rabbit IgG (H&L) - Alexa Fluor 594 Secondary antibody was diluted at 1:1000 (room temperature, 50min).3, DAPI (blue) 10min.
ICC/IF
Immunofluorescence analysis of Hela cell. 1,NOS3 (phospho Ser1177) Antibody (green) was diluted at 1:200 (4℃ overnight). 2, Goat Anti Rabbit Alexa Fluor 488 was diluted at 1:1000 (room temperature, 50min). 3 DAPI (blue) 10min.| Application Notes | WB:1:2000-1:10000 IHC:1:200-1:1000 ICC/IF:1:200-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year(Do not lower than -25°C). |
| Storage Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA. |
