ELISA
Coat the plate with 100ng immunizing antigen. The phospho-specific antibody,pbs only can specifically recognize phosphorylated peptide and enable quantitative detection over a wide dynamic range. Non-phosphorylated peptide shows no cross-reactivity, and the background signal remains low, indicating that the phospho-specific antibody,pbs only possesses high specificity and an excellent signal-to-noise ratio. Black line: phospho-peptide; Rad line: non-phospho peptide| Product Name | Anti-Phospho-eNOS(Ser1176) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide conjugated with KLH. |
| Modification | p-Ser1176 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISA |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NOS3 |
|---|---|
| Gene Synonyms | cNOS eNOS ECNOS MYMY8 EC-NOS NOSIII |
| Gene Full Name | nitric oxide synthase 3 |
| Gene Summary | Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Alternative splicing and the use of alternative promoters results in multiple transcript variants. [provided by RefSeq, Oct 2016] |
| Alternative Names | cNOS Constitutive NOS EC NOS EC-NOS ecNOS Endothelial nitric oxidase synthase Endothelial nitric oxide synthase Endothelial nitric oxide synthase 3 Endothelial NOS eNOS Nitric oxide synthase 3 (endothelial cell) Nitric oxide synthase 3 Nitric oxide synthase 3 endothelial cell Nitric oxide synthase endothelial Nitric oxide synthase endothelial NOS 3 NOS III NOS type III NOS3 NOS3_HUMAN NOSIII |
| Molecular Weight(MW) | 133kDa |
| Cellular Localization | Cell membrane, Cytoplasm, Cytoskeleton, Golgi apparatus, Membrane |
| SwissProt ID | P29474 |
|---|
ELISA
Coat the plate with 100ng immunizing antigen. The phospho-specific antibody,pbs only can specifically recognize phosphorylated peptide and enable quantitative detection over a wide dynamic range. Non-phosphorylated peptide shows no cross-reactivity, and the background signal remains low, indicating that the phospho-specific antibody,pbs only possesses high specificity and an excellent signal-to-noise ratio. Black line: phospho-peptide; Rad line: non-phospho peptide| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide,40% Glycerol. |
