WB
Western blot analysis of PEX5L using anti-PEX5L antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-PEX5L antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.IHC
IHC analysis of PEX5L using anti-PEX5L antibody. PEX5L was detected in a paraffin-embedded section of human thyroid cancer tissue. The tissue section was incubated with rabbit anti-PEX5L Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
IHC analysis of PEX5L using anti-PEX5L antibody. PEX5L was detected in a paraffin-embedded section of mouse brain tissue. The tissue section was incubated with rabbit anti-PEX5L Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
IHC analysis of PEX5L using anti-PEX5L antibody. PEX5L was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-PEX5L Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IF-P
IF analysis of PEX5L using anti-PEX5L antibody. PEX5L was detected in a paraffin-embedded section of human testis tissue. The tissue section was incubated with rabbit anti-PEX5L Antibody at a dilution of 1:100. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red) was used as secondary antibody. The section was counterstained with DAPI (Blue).IF-P
IF analysis of PEX5L using anti-PEX5L antibody. PEX5L was detected in a paraffin-embedded section of mouse brain tissue. The tissue section was incubated with rabbit anti-PEX5L Antibody at a dilution of 1:100. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red) was used as secondary antibody. The section was counterstained with DAPI (Blue).FC
Flow Cytometry analysis of U251 cells using anti-PEX5L antibody. Overlay histogram showing U251 cells stained with Rabbit polyclonal antibody to PEX5L (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PEX5L Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Product Name | Rabbit polyclonal antibody to PEX5L |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human PEX5L. Human PEX5L shares 95.2% amino acid (aa) sequence identity with mouse PEX5L. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCIF-PIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:400 IF-P:1:50-1:400 FC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PEX5L |
|---|---|
| Gene Synonyms | PXR2 PEX5R PXR2B PEX5RP TRIP8b |
| Gene Full Name | peroxisomal biogenesis factor 5 like |
| Gene Summary | Enables peroxisome matrix targeting signal-1 binding activity and small GTPase binding activity. Predicted to be involved in protein import into peroxisome matrix, docking. Predicted to act upstream of or within maintenance of protein location and regulation of membrane potential. Located in cytosol. Part of receptor complex. [provided by Alliance of Genome Resources, Jul 2025] |
| Alternative Names | Peroxin 5 related protein PEX2 related protein PEX5 like protein PEX5 related protein PEX5L PEX5R Pex5Rp PXR2 PXR2B |
| Molecular Weight(MW) | 65 kDa |
| Cellular Localization | Cytoplasm,Membrane. |
WB
Western blot analysis of PEX5L using anti-PEX5L antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-PEX5L antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
IHC
IHC analysis of PEX5L using anti-PEX5L antibody. PEX5L was detected in a paraffin-embedded section of human thyroid cancer tissue. The tissue section was incubated with rabbit anti-PEX5L Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of PEX5L using anti-PEX5L antibody. PEX5L was detected in a paraffin-embedded section of mouse brain tissue. The tissue section was incubated with rabbit anti-PEX5L Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of PEX5L using anti-PEX5L antibody. PEX5L was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-PEX5L Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IF-P
IF analysis of PEX5L using anti-PEX5L antibody. PEX5L was detected in a paraffin-embedded section of human testis tissue. The tissue section was incubated with rabbit anti-PEX5L Antibody at a dilution of 1:100. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red) was used as secondary antibody. The section was counterstained with DAPI (Blue).
IF-P
IF analysis of PEX5L using anti-PEX5L antibody. PEX5L was detected in a paraffin-embedded section of mouse brain tissue. The tissue section was incubated with rabbit anti-PEX5L Antibody at a dilution of 1:100. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red) was used as secondary antibody. The section was counterstained with DAPI (Blue).
FC
Flow Cytometry analysis of U251 cells using anti-PEX5L antibody. Overlay histogram showing U251 cells stained with Rabbit polyclonal antibody to PEX5L (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PEX5L Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 IF-P:1:50-1:400 FC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| Storage Buffer | 500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
