WB
Positive control:MOLT-4, Raji, MCF-7, T47D, SK-BR-3, Hela cell lysates . Recommended dilution: 500-2000. Gel: 8%SDS-PAGE. Lysate: 20 μg. Lane 1-6: MOLT-4, Raji, MCF-7, T47D, SK-BR-3, Hela cell lysates. Primary antibody: ESR1 Antibody at dilution 1/1000. Secondary antibody: HRP-conjugated Goat anti mouse IgG at 1/10000 dilution. Exposure time: 2 minutes.IHC
Predicted cell location: Nucleus. Positive control: Human breast cancer. Recommended dilution: 200-1000. The image is immunohistochemistry of paraffin-embedded Human breast cancer tissue using ESR1 Antibody at dilution 1/800.ICC/IF
Predicted cell location: Nucleus. Positive control: Hela cells. Recommended dilution: 200-1000. The image is immunofluorescence of Hela cells using ESR1 Antibody at dilution 1/200 (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.FC
The image is flow cytometry analysis of Hela cells using ESR1 antibody at dilution 1/400 (green) and negative control (red).| Product Name | Anti-ESR1 mouse monoclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Fusion protein of human ESR1. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | FCICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:200-1:1000 ICC/IF:1:200-1:1000 FC:1:200-1:400 |
|
| Species Reactivity | HumanRat |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Gene Symbol | ESR1 |
|---|---|
| Gene Synonyms | ER ESR Era ESRA ESTRR NR3A1 |
| Gene Full Name | estrogen receptor 1 |
| Gene Summary | This gene encodes an estrogen receptor and ligand-activated transcription factor. The canonical protein contains an N-terminal ligand-independent transactivation domain, a central DNA binding domain, a hinge domain, and a C-terminal ligand-dependent transactivation domain. The protein localizes to the nucleus where it may form either a homodimer or a heterodimer with estrogen receptor 2. The protein encoded by this gene regulates the transcription of many estrogen-inducible genes that play a role in growth, metabolism, sexual development, gestation, and other reproductive functions and is expressed in many non-reproductive tissues. The receptor encoded by this gene plays a key role in breast cancer, endometrial cancer, and osteoporosis. This gene is reported to have dozens of transcript variants due to the use of alternate promoters and alternative splicing, however, the full-length nature of many of these variants remain uncertain. [provided by RefSeq, Jul 2020] |
| Alternative Names | estrogen receptor 1 ER ESR Era ESRA ESTRR NR3A1 |
| Molecular Weight(MW) | 66 kDa |
| Cellular Localization | Cell membrane,Cytoplasm,Golgi apparatus,Membrane,Nucleus. |
WB
Positive control:MOLT-4, Raji, MCF-7, T47D, SK-BR-3, Hela cell lysates . Recommended dilution: 500-2000. Gel: 8%SDS-PAGE. Lysate: 20 μg. Lane 1-6: MOLT-4, Raji, MCF-7, T47D, SK-BR-3, Hela cell lysates. Primary antibody: ESR1 Antibody at dilution 1/1000. Secondary antibody: HRP-conjugated Goat anti mouse IgG at 1/10000 dilution. Exposure time: 2 minutes.
IHC
Predicted cell location: Nucleus. Positive control: Human breast cancer. Recommended dilution: 200-1000. The image is immunohistochemistry of paraffin-embedded Human breast cancer tissue using ESR1 Antibody at dilution 1/800.
ICC/IF
Predicted cell location: Nucleus. Positive control: Hela cells. Recommended dilution: 200-1000. The image is immunofluorescence of Hela cells using ESR1 Antibody at dilution 1/200 (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
FC
The image is flow cytometry analysis of Hela cells using ESR1 antibody at dilution 1/400 (green) and negative control (red).| Application Notes | WB:1:500-1:2000 IHC:1:200-1:1000 ICC/IF:1:200-1:1000 FC:1:200-1:400 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -20°C. |
| Storage Buffer | PBS (PH 7.4) containing 0.05% sodium azide. |
