WB
Western blot analysis of PFKP using anti-PFKP antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-PFKP antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.IHC
IHC analysis of PFKP using anti-PFKP antibody. PFKP was detected in a paraffin-embedded section of human breast cancer tissue. The tissue section was incubated with rabbit anti-PFKP Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
IHC analysis of PFKP using anti-PFKP antibody. PFKP was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-PFKP Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
IHC analysis of PFKP using anti-PFKP antibody. PFKP was detected in a paraffin-embedded section of mouse brain tissue. The tissue section was incubated with rabbit anti-PFKP Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.ICC/IF
ICC/IF analysis of PFKP using anti-PFKP antibody. PFKP was detected in an immunocytochemical section of A549 cells. The section was incubated with rabbit anti-PFKP Antibody at a dilution of 1:100. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red) was used as secondary antibody. The section was counterstained with DAPI (Blue).| Product Name | Rabbit polyclonal antibody to PFKP |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human PFKP recombinant protein (Position: G440-V784). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PFKP |
|---|---|
| Gene Synonyms | PFKF PFK-C PFK-P ATP-PFK |
| Gene Full Name | phosphofructokinase, platelet |
| Gene Summary | This gene encodes a member of the phosphofructokinase A protein family. The encoded enzyme is the platelet-specific isoform of phosphofructokinase and plays a key role in glycolysis regulation. This gene may play a role in metabolic reprogramming in some cancers, including clear cell renal cell carcinomas, and cancer of the bladder, breast, and lung. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2016] |
| Alternative Names | 6 phosphofructokinase type C PFK C PFKF PFKP Phosphofructokinase 1 phosphofructokinase platelet Phosphohexokinase |
| Molecular Weight(MW) | 86 kDa |
| Cellular Localization | Cytoplasm. |
WB
Western blot analysis of PFKP using anti-PFKP antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-PFKP antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
IHC
IHC analysis of PFKP using anti-PFKP antibody. PFKP was detected in a paraffin-embedded section of human breast cancer tissue. The tissue section was incubated with rabbit anti-PFKP Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of PFKP using anti-PFKP antibody. PFKP was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-PFKP Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of PFKP using anti-PFKP antibody. PFKP was detected in a paraffin-embedded section of mouse brain tissue. The tissue section was incubated with rabbit anti-PFKP Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
ICC/IF
ICC/IF analysis of PFKP using anti-PFKP antibody. PFKP was detected in an immunocytochemical section of A549 cells. The section was incubated with rabbit anti-PFKP Antibody at a dilution of 1:100. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red) was used as secondary antibody. The section was counterstained with DAPI (Blue).| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt，-20℃ as supplied. |
| Storage Buffer | 500 ug/ml antibody with PBS，0.02% NaN3, 1 mg BSA and 50% glycerol. |
