WB
Western blot analysis of PRAG1 using anti-PRAG1 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-PRAG1 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.ICC/IF
ICC/IF analysis of PRAG1 using anti-PRAG1 antibody. PRAG1 was detected in an immunocytochemical section of Hela cells. The section was incubated with rabbit anti-PRAG1 Antibody at a dilution of 1:100. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red) was used as secondary antibody. The section was counterstained with DAPI (Blue).FC
Flow Cytometry analysis of HepG2 cells using anti-PRAG1 antibody. Overlay histogram showing HepG2 cells stained with Rabbit polyclonal antibody to PRAG1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PRAG1 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample (Red line) was also used as a control.| Product Name | Rabbit polyclonal antibody to PRAG1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human PRAG1 recombinant protein (Position: H2-V1104). Human PRAG1 shares 74.1% and 73.4% amino acid (aa) sequence identity with mouse and rat PRAG1, respectively. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFWB |
| WB:1:500-1:2000 ICC/IF:1:50-1:400 FC:1:50-1:200 |
|
| Species Reactivity | Human |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PRAG1 |
|---|---|
| Gene Synonyms | NACK PEAK2 SGK223 PRAGMIN |
| Gene Full Name | PEAK1 related, kinase-activating pseudokinase 1 |
| Gene Summary | This gene encodes an enzyme that belongs to the tyrosine protein kinase family. A similar protein in rat binds to Rho family GTPase 2 (Rnd2) and regulates neurite outgrowth via activation of Ras homolog gene family, member A (RhoA). [provided by RefSeq, Mar 2014] |
| Alternative Names | PRAG1 SGK223 Inactive tyrosine-protein kinase PRAG1 PEAK1-related kinase-activating pseudokinase 1 Pragmin Sugen kinase 223 SgK223 |
| Molecular Weight(MW) | 150 kDa(Observed band size:200 kDa) |
| Cellular Localization | Cell junction,Cytoplasm,Nucleus. |
WB
Western blot analysis of PRAG1 using anti-PRAG1 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-PRAG1 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
ICC/IF
ICC/IF analysis of PRAG1 using anti-PRAG1 antibody. PRAG1 was detected in an immunocytochemical section of Hela cells. The section was incubated with rabbit anti-PRAG1 Antibody at a dilution of 1:100. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red) was used as secondary antibody. The section was counterstained with DAPI (Blue).
FC
Flow Cytometry analysis of HepG2 cells using anti-PRAG1 antibody. Overlay histogram showing HepG2 cells stained with Rabbit polyclonal antibody to PRAG1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PRAG1 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample (Red line) was also used as a control.| Application Notes | WB:1:500-1:2000 ICC/IF:1:50-1:400 FC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt，-20℃ as supplied. |
| Storage Buffer | 500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
