WB
Western blot analysis of VGLUT1 on different lysates with Guinea pig anti-VGLUT1 antibody at 1/5,000 dilution. Notice: no heat means the lysate is not boiled. Exposure time: 6 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in primary antibody dilution at 4℃ overnight. Rabbit anti-Guinea pig IgG H&L - HRP Secondary Antibody at 1/5,000 dilution was used for 1 hour at room temperature.IF-F
Application: IHC-Fr. Species: Mouse. Site: hippocampus. Sample: Frozen section. Antibody concentration: 1/500 (VGLUT1, Guinea pig, red); 1/500 (VGLUT2, Mouse, green). Antigen retrieval: Not required.IF-F
Application: IHC-Fr. Species: Rat. Site: hippocampus. Sample: Frozen section. Antibody concentration: 1/500 (VGLUT1, Guinea pig, red); 1/500 (VGLUT2, Mouse, green). Antigen retrieval: Not required.IF-P
Application: Immunofluorescence (IF-tissue). Species: Mouse. Tissue: Hippocampus. Sample: Paraffin-embedded section. Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃. Wash buffer: 1× PBST. Endogenous peroxidase blocking: 3% H2O2, 10 minutes. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: VGLUT1, 1/500 (Guinea pig, Red), GAD67, 1:500 (Mouse, Green), overnight at 4℃. Secondary antibody: Goat Anti-Mouse IgG (488), Goat anti-Guinea pig IgG (594), 1.5 hours at room temperature.IF-P
Application: Immunofluorescence (IF-tissue). Species: Rat. Tissue: Hippocampus. Sample: Paraffin-embedded section. Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃. Wash buffer: 1× PBST. Endogenous peroxidase blocking: 3% H2O2, 10 minutes. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: VGLUT1, 1/500 (Guinea pig, Red), GAD67, 1:500 (Mouse, Green), overnight at 4℃. Secondary antibody: Goat Anti-Mouse IgG (488), Goat anti-Guinea pig IgG (594), 1.5 hours at room temperature.| Product Name | VGLUT1 Recombinant Antibody - Guinea pig IgG2 (Chimeric) |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within Mouse VGLUT1 aa 1-560. |
| Isotype | IgG2 |
|---|---|
| Tested Applications | IF-FIF-PWB |
| WB:1:5000 IF-F:1:500 IF-P:1:500 |
|
| Species Reactivity | MouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | SLC17A7 |
|---|---|
| Gene Synonyms | BNPI VGLUT1 |
| Gene Full Name | solute carrier family 17 member 7 |
| Gene Summary | The protein encoded by this gene is a vesicle-bound, sodium-dependent phosphate transporter that is specifically expressed in the neuron-rich regions of the brain. It is preferentially associated with the membranes of synaptic vesicles and functions in glutamate transport. The protein shares 82% identity with the differentiation-associated Na-dependent inorganic phosphate cotransporter and they appear to form a distinct class within the Na+/Pi cotransporter family. [provided by RefSeq, Jul 2008] |
| Alternative Names | BNPI antibody Brain specific Na (+) dependent inorganic phosphate cotransporter antibody Brain specific Na dependent inorganic phosphate cotransporter antibody Brain-specific Na(+)-dependent inorganic phosphate cotransporter antibody Slc17a7 antibody Solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter) member 7 antibody Solute carrier family 17 member 7 antibody Vesicular glutamate transporter 1 antibody VGLU1_HUMAN antibody VGLUT 1 antibody VGluT1 antibody |
| Molecular Weight(MW) | 62 kDa |
| Cellular Localization | Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane, Cell membrane, Synapse, synaptosome. |
WB
Western blot analysis of VGLUT1 on different lysates with Guinea pig anti-VGLUT1 antibody at 1/5,000 dilution. Notice: no heat means the lysate is not boiled. Exposure time: 6 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in primary antibody dilution at 4℃ overnight. Rabbit anti-Guinea pig IgG H&L - HRP Secondary Antibody at 1/5,000 dilution was used for 1 hour at room temperature.
IF-F
Application: IHC-Fr. Species: Mouse. Site: hippocampus. Sample: Frozen section. Antibody concentration: 1/500 (VGLUT1, Guinea pig, red); 1/500 (VGLUT2, Mouse, green). Antigen retrieval: Not required.
IF-F
Application: IHC-Fr. Species: Rat. Site: hippocampus. Sample: Frozen section. Antibody concentration: 1/500 (VGLUT1, Guinea pig, red); 1/500 (VGLUT2, Mouse, green). Antigen retrieval: Not required.
IF-P
Application: Immunofluorescence (IF-tissue). Species: Mouse. Tissue: Hippocampus. Sample: Paraffin-embedded section. Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃. Wash buffer: 1× PBST. Endogenous peroxidase blocking: 3% H2O2, 10 minutes. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: VGLUT1, 1/500 (Guinea pig, Red), GAD67, 1:500 (Mouse, Green), overnight at 4℃. Secondary antibody: Goat Anti-Mouse IgG (488), Goat anti-Guinea pig IgG (594), 1.5 hours at room temperature.
IF-P
Application: Immunofluorescence (IF-tissue). Species: Rat. Tissue: Hippocampus. Sample: Paraffin-embedded section. Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃. Wash buffer: 1× PBST. Endogenous peroxidase blocking: 3% H2O2, 10 minutes. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: VGLUT1, 1/500 (Guinea pig, Red), GAD67, 1:500 (Mouse, Green), overnight at 4℃. Secondary antibody: Goat Anti-Mouse IgG (488), Goat anti-Guinea pig IgG (594), 1.5 hours at room temperature.| Application Notes | WB:1:5000 IF-F:1:500 IF-P:1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage Buffer | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
