WB
Western blot analysis of various lysates using VGLUT1 Rabbit mAb at 1:1000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Negative control (NC): Rat lung Exposure time: 45s.IHC
Immunohistochemistry analysis of paraffin embedded Mouse brain tissue using VGLUT1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.IF-P
Confocal imaging of paraffin-embedded Mouse cerebellum tissue using VGLUT1 Rabbit mAb (dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Microwave antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining. objective: 40x.| Product Name | VGLUT1 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthetic peptide corresponding to a sequence within amino acids 461-560 of human VGLUT1 (NP_064705.1). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IF-PIHCWB |
| WB:1:1000-1:3000 IHC:1:200-1:800 IF-P:1:200-1:2000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SLC17A7 |
|---|---|
| Gene Synonyms | BNPI VGLUT1 |
| Gene Full Name | solute carrier family 17 member 7 |
| Gene Summary | The protein encoded by this gene is a vesicle-bound, sodium-dependent phosphate transporter that is specifically expressed in the neuron-rich regions of the brain. It is preferentially associated with the membranes of synaptic vesicles and functions in glutamate transport. The protein shares 82% identity with the differentiation-associated Na-dependent inorganic phosphate cotransporter and they appear to form a distinct class within the Na+/Pi cotransporter family. [provided by RefSeq, Jul 2008] |
| Alternative Names | BNPI VGLUT1 |
| Molecular Weight(MW) | 62kDa |
| Cellular Localization | excitatory synapse,neuron projection,plasma membrane,presynaptic active zone,synaptic vesicle membrane,Cell junction,Cytoplasmic vesicle,Membrane,Multi-pass membrane protein,secretory vesicle,synapse,synaptosome. |
WB
Western blot analysis of various lysates using VGLUT1 Rabbit mAb at 1:1000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Negative control (NC): Rat lung Exposure time: 45s.
IHC
Immunohistochemistry analysis of paraffin embedded Mouse brain tissue using VGLUT1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.
IF-P
Confocal imaging of paraffin-embedded Mouse cerebellum tissue using VGLUT1 Rabbit mAb (dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Microwave antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining. objective: 40x.| Application Notes | WB:1:1000-1:3000 IHC:1:200-1:800 IF-P:1:200-1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | Buffer: 10 mM sodium HEPES and 150 mM NaCl with 0.02% Sodium azide,100 μg/ml BSA,50% Glycerol,pH 7.5. |
