WB
Western blot analysis of VGluT1 on different lysates with Rabbit anti-VGluT1 antibody at 1/2,000 dilution. Lane 1: Mouse brain tissue lysate (no heat)， Lane 2: Mouse lung tissue lysate (no heat) (negative)， Lane 3: Rat brain tissue lysate (no heat)， Lane 4: Rat lung tissue lysate (no heat) (negative)， Notice: no heat means the lysate is not boiled. Lysates/proteins at 20 µg/Lane. Exposure time: 8 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-VGluT1 antibody at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IF-F
Species: Rat, Site: Cerebellum, Sample: Frozen section, Antibody concentration: 1:500, Antigen retrieval: Not required.| Product Name | VGluT1 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IF-FIHCWB |
| WB:1:2000 IHC:1:500-1:2000 IF-F:1:500-1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | SLC17A7 |
|---|---|
| Gene Synonyms | BNPI VGLUT1 |
| Gene Full Name | solute carrier family 17 member 7 |
| Gene Summary | The protein encoded by this gene is a vesicle-bound, sodium-dependent phosphate transporter that is specifically expressed in the neuron-rich regions of the brain. It is preferentially associated with the membranes of synaptic vesicles and functions in glutamate transport. The protein shares 82% identity with the differentiation-associated Na-dependent inorganic phosphate cotransporter and they appear to form a distinct class within the Na+/Pi cotransporter family. [provided by RefSeq, Jul 2008] |
| Alternative Names | BNPI antibody Brain specific Na (+) dependent inorganic phosphate cotransporter antibody Brain specific Na dependent inorganic phosphate cotransporter antibody Brain-specific Na(+)-dependent inorganic phosphate cotransporter antibody Slc17a7 antibody Solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter) member 7 antibody Solute carrier family 17 member 7 antibody Vesicular glutamate transporter 1 antibody VGLU1_HUMAN antibody VGLUT 1 antibody BNPI antibody Brain specific Na (+) dependent inorganic phosphate cotransporter antibody Brain specific Na dependent inorganic phosphate cotransporter antibody Brain-specific Na(+)-dependent inorganic phosphate cotransporter antibody Slc17a7 antibody Solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter) member 7 antibody Solute carrier family 17 member 7 antibody Vesicular glutamate transporter 1 antibody VGLU1_HUMAN antibody VGLUT 1 antibody VGluT1 antibody |
| Molecular Weight(MW) | 62kDa |
| Cellular Localization | Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane, Cell membrane, Synapse, synaptosome. |
WB
Western blot analysis of VGluT1 on different lysates with Rabbit anti-VGluT1 antibody at 1/2,000 dilution. Lane 1: Mouse brain tissue lysate (no heat)， Lane 2: Mouse lung tissue lysate (no heat) (negative)， Lane 3: Rat brain tissue lysate (no heat)， Lane 4: Rat lung tissue lysate (no heat) (negative)， Notice: no heat means the lysate is not boiled. Lysates/proteins at 20 µg/Lane. Exposure time: 8 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-VGluT1 antibody at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IF-F
Species: Rat, Site: Cerebellum, Sample: Frozen section, Antibody concentration: 1:500, Antigen retrieval: Not required.| Application Notes | WB:1:2000 IHC:1:500-1:2000 IF-F:1:500-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
