WB
Western blot analysis of ND3/MT-ND3 using anti-ND3/MT-ND3 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ND3/MT-ND3 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.| Product Name | Rabbit polyclonal antibody to ND3/MT-ND3 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of mouse ND3/MT-ND3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-1:2000 |
|
| Species Reactivity | MouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | mt-Nd3 |
|---|---|
| Gene Synonyms | ND3 |
| Gene Full Name | NADH dehydrogenase 3, mitochondrial |
| Gene Summary | Predicted to enable NADH dehydrogenase (ubiquinone) activity. Predicted to be involved in cellular response to glucocorticoid stimulus; mitochondrial electron transport, NADH to ubiquinone; and response to oxidative stress. Located in mitochondrion. Part of respiratory chain complex I. Is active in mitochondrial inner membrane. Is expressed in several structures, including alimentary system; brain; genitourinary system; hemolymphoid system gland; and liver and biliary system. Human ortholog(s) of this gene implicated in Leber hereditary optic neuropathy; Leigh disease; and Parkinson's disease. Orthologous to human MT-ND3 (mitochondrially encoded NADH:ubiquinone oxidoreductase core subunit 3). [provided by Alliance of Genome Resources, Jul 2025] |
| Alternative Names | MT ND3 MTND3 NADH dehydrogenase subunit 3 NADH3 ND3 |
| Molecular Weight(MW) | 13 kDa(Observed band size:15 kDa) |
| Cellular Localization | Membrane,Mitochondrion,Mitochondrion inner membrane. |
WB
Western blot analysis of ND3/MT-ND3 using anti-ND3/MT-ND3 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ND3/MT-ND3 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.| Application Notes | WB:1:500-1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| Storage Buffer | 500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
