WB
Western blot analysis of anti-MISP antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-MISP antigen affinity purified polyclonal antibody and probed with a goat anti-rabbit IgG-HRP secondary antibody . The signal is developed using ECL Plus Western Blotting Substrate.ICC/IF
ICC/IF analysis of MISP using anti-MISP antibody. MISP was detected in an immunocytochemical section of U2OS cells. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red) was used as secondary antibody. The section was counterstained with DAPI (Blue).FC
Flow Cytometry analysis of Caco-2 cells using anti-MISP antibody. Overlay histogram showing Caco-2 cells stained with Rabbit polyclonal antibody to MISP (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MISP Antibody (1:100). Fluoro488 conjugated goat anti-rabbit IgG (1:100) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.| Product Name | Rabbit polyclonal antibody to MISP |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E. coli-derived human MISP recombinant protein (Position: M1-R632). Human MISP shares 62% amino acid (aa) sequence identity with mouse MISP. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFWB |
| WB:1:500-1:2000 ICC/IF:1:50-1:400 FC:1:50-1:200 |
|
| Species Reactivity | Human |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PRR9 |
|---|---|
| Gene Full Name | proline rich 9 |
| Alternative Names | Sik1 Salt-inducible kinase 1 salt inducible kinase 1 Myocardial SNF1-like kinase Msk |
| Molecular Weight(MW) | 75 kDa |
| Cellular Localization | Cell junction,Cytoplasm,Cytoskeleton. |
WB
Western blot analysis of anti-MISP antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-MISP antigen affinity purified polyclonal antibody and probed with a goat anti-rabbit IgG-HRP secondary antibody . The signal is developed using ECL Plus Western Blotting Substrate.
ICC/IF
ICC/IF analysis of MISP using anti-MISP antibody. MISP was detected in an immunocytochemical section of U2OS cells. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red) was used as secondary antibody. The section was counterstained with DAPI (Blue).
FC
Flow Cytometry analysis of Caco-2 cells using anti-MISP antibody. Overlay histogram showing Caco-2 cells stained with Rabbit polyclonal antibody to MISP (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MISP Antibody (1:100). Fluoro488 conjugated goat anti-rabbit IgG (1:100) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.| Application Notes | WB:1:500-1:2000 ICC/IF:1:50-1:400 FC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt，-20℃ as supplied. |
| Storage Buffer | 500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
