WB
Western blot analysis of eIF3g on different lysates with Rabbit anti-eIF3g antibody at 1/25,000 dilution. Lysates/proteins at 20 µg/Lane. Exposure time: 4 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/25,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.ICC/IF
Immunocytochemistry analysis of HeLa cells labeling eIF3g with Rabbit anti-eIF3g antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-eIF3g antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (594) was used as the secondary antibody at 1/1,000 dilution.FC
Flow cytometric analysis of HeLa cells labeling eIF3g. Cells were fixed and permeabilized. Then stained with the primary antibody (1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).IP
eIF3g was immunoprecipitated from 0.2 mg SH-SY5Y cell lysate with eIF3g Recombinant Rabbit Monoclonal Antibody at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using eIF3g Recombinant Rabbit Monoclonal Antibody at 1/5,000 dilution. Mouse anti Rabbit IgG heavy chain (Fc) secondary antibody at 1/10,000 dilution was used for 1 hour at room temperature. Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 12 seconds.| Product Name | eIF3g Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within human eIF3g aa 1-320. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIPWB |
| WB:1:25000 ICC/IF:1:100 FC:1:1000 IP:1-2μg/sample |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | EIF3G |
|---|---|
| Gene Synonyms | EIF3S4 EIF3-P42 eIF3-p44 eIF3-delta |
| Gene Full Name | eukaryotic translation initiation factor 3 subunit G |
| Gene Summary | This gene encodes a core subunit of the eukaryotic translation initiation factor 3 (eIF3) complex, which is required for initiation of protein translation. An N-terminal caspase cleavage product of the encoded protein may stimulate degradation of DNA. A mutation in this gene is associated with narcolepsy. [provided by RefSeq, Jul 2016] |
| Alternative Names | 44kDa antibody Anti EIF3S4 antibody eIF 3 delta antibody eIF 3 RNA binding subunit antibody eIF-3 RNA-binding subunit antibody eIF-3-delta antibody eIF3 delta antibody eIF3 p42 antibody eIF3 p44 antibody eIF3g antibody EIF3G_HUMAN antibody EIF3S4 antibody EIF3S4 antibody antibody EIF3S4 formerly antibody Eukaryotic translation initiation factor 3 antibody Eukaryotic translation initiation factor 3 RNA binding subunit antibody Eukaryotic translation initiation factor 3 RNA-binding subunit antibody Eukaryotic translation initiation factor 3 subunit 4 antibody Eukaryotic translation initiation factor 3 subunit 4 delta 44 kD antibody Eukaryotic translation initiation factor 3 subunit G antibody Eukaryotic translation initiation factor 3 subunit p42 antibody Eukaryotic translation initiation factor 3 subunit 4 formerly antibody Subunit 4 (delta 44kD) antibody Subunit 4 delta antibody |
| Molecular Weight(MW) | 36 kDa(Observed band size:45 kDa) |
| Cellular Localization | Cytoplasm, Nucleus, perinuclear region. |
WB
Western blot analysis of eIF3g on different lysates with Rabbit anti-eIF3g antibody at 1/25,000 dilution. Lysates/proteins at 20 µg/Lane. Exposure time: 4 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/25,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
ICC/IF
Immunocytochemistry analysis of HeLa cells labeling eIF3g with Rabbit anti-eIF3g antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-eIF3g antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (594) was used as the secondary antibody at 1/1,000 dilution.
FC
Flow cytometric analysis of HeLa cells labeling eIF3g. Cells were fixed and permeabilized. Then stained with the primary antibody (1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
IP
eIF3g was immunoprecipitated from 0.2 mg SH-SY5Y cell lysate with eIF3g Recombinant Rabbit Monoclonal Antibody at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using eIF3g Recombinant Rabbit Monoclonal Antibody at 1/5,000 dilution. Mouse anti Rabbit IgG heavy chain (Fc) secondary antibody at 1/10,000 dilution was used for 1 hour at room temperature. Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 12 seconds.| Application Notes | WB:1:25000 ICC/IF:1:100 FC:1:1000 IP:1-2μg/sample |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
