Application
EIF3G Antibody (Center) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the EIF3G antibody detected the EIF3G protein (arrow).Application
EIF3G Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human cervix carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the EIF3G Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.Application
EIF3G Antibody (Center) flow cytometric analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | EIF3G Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | EIF3S4 |
|---|---|
| Alternative Names | EIF3G EIF3S4 Eukaryotic translation initiation factor 3 subunit G Eukaryotic translation initiation factor 3 RNA-binding subunit Eukaryotic translation initiation factor 3 subunit 4 eIF-3-delta eIF3 p42 eIF3 p44 |
| Molecular Weight(MW) | 35611 Da |
| Function | Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. This subunit can bind 18S rRNA |
| Tissue Specificity | This EIF3G antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 217-247 amino acids from the Central region of human EIF3G. |
| Cellular Localization | Cytoplasm (By similarity). Nucleus. Cytoplasm, perinuclear region. Note=Colocalizes with AIFM1 in the nucleus and perinuclear region |
| Entrez Gene | 8666 |
|---|---|
| Nucleotide Accession | NP_003746.2 |
Application
EIF3G Antibody (Center) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the EIF3G antibody detected the EIF3G protein (arrow).
Application
EIF3G Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human cervix carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the EIF3G Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
EIF3G Antibody (Center) flow cytometric analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
