WB
Western blot analysis of CD130 / GP130 on different lysates with Rabbit anti-CD130 / GP130 antibody at 1/5,000 dilution. Lysates/proteins at 20 µg/Lane. Exposure time: 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.WB
Western blot analysis of CD130 / GP130 on different lysates with Rabbit anti-CD130 / GP130 antibody at 1/5,000 dilution. Lysates/proteins at 30 µg/Lane. Exposure time: 18 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.| Product Name | CD130 / GP130 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within mouse CD130 / GP130 aa 1-617. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:5000 |
|
| Species Reactivity | Mouse |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | IL6ST |
|---|---|
| Gene Synonyms | CD130 GP130 HIES4 IMD94 STWS2 CDW130 HIES4A HIES4B IL-6RB sGP130 |
| Gene Full Name | interleukin 6 cytokine family signal transducer |
| Gene Summary | The protein encoded by this gene is a signal transducer shared by many cytokines, including interleukin 6 (IL6), ciliary neurotrophic factor (CNTF), leukemia inhibitory factor (LIF), and oncostatin M (OSM). This protein functions as a part of the cytokine receptor complex. The activation of this protein is dependent upon the binding of cytokines to their receptors. vIL6, a protein related to IL6 and encoded by the Kaposi sarcoma-associated herpesvirus, can bypass the interleukin 6 receptor (IL6R) and directly activate this protein. Knockout studies in mice suggest that this gene plays a critical role in regulating myocyte apoptosis. Alternatively spliced transcript variants have been described. A related pseudogene has been identified on chromosome 17. [provided by RefSeq, May 2014] |
| Alternative Names | CD130 antibody CD130 antigen antibody CDw130 antibody gp130 antibody GP130 RAPS antibody IL 6R beta antibody IL-6 receptor subunit beta antibody IL-6R subunit beta antibody IL-6R-beta antibody IL-6RB antibody IL6 ST antibody IL6RB_HUMAN antibody IL6ST antibody Interleukin 6 receptor subunit beta antibody Interleukin receptor beta chain antibody Interleukin-6 receptor subunit beta antibody Interleukin-6 signal transducer antibody Membrane glycoprotein 130 antibody Membrane glycoprotein gp130 antibody Oncostatin M receptor antibody Oncostatin M receptor alpha subunit antibody Oncostatin-M receptor subunit alpha antibody |
| Molecular Weight(MW) | 102 kDa(Observed band size:130 kDa) |
| Cellular Localization | Cell membrane. |
WB
Western blot analysis of CD130 / GP130 on different lysates with Rabbit anti-CD130 / GP130 antibody at 1/5,000 dilution. Lysates/proteins at 20 µg/Lane. Exposure time: 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
WB
Western blot analysis of CD130 / GP130 on different lysates with Rabbit anti-CD130 / GP130 antibody at 1/5,000 dilution. Lysates/proteins at 30 µg/Lane. Exposure time: 18 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.| Application Notes | WB:1:5000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage Buffer | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
