WB
25 ug total protein per lane of various lysates (see on figure) probed with GRM2 monoclonal antibody, unconjugated at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.IHC
Paraformaldehyde-fixed, paraffin embedded Human Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GRM2 Monoclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by conjugation to HRP‑conjugated secondary antibody and DAB staining.IHC
Paraformaldehyde-fixed, paraffin embedded Rat Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GRM2 Monoclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by conjugation to HRP‑conjugated secondary antibody and DAB staining.| Product Name | GRM2 | mGluR2 Recombinant Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthesized peptide derived from human mGluR2 : 800-872. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:1000-5000 IHC:1:100-500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | GRM2 |
|---|---|
| Gene Synonyms | GLUR2 mGlu2 GPRC1B MGLUR2 |
| Gene Full Name | glutamate metabotropic receptor 2 |
| Gene Summary | L-glutamate is the major excitatory neurotransmitter in the central nervous system and activates both ionotropic and metabotropic glutamate receptors. Glutamatergic neurotransmission is involved in most aspects of normal brain function and can be perturbed in many neuropathologic conditions. The metabotropic glutamate receptors are a family of G protein-coupled receptors, that have been divided into 3 groups on the basis of sequence homology, putative signal transduction mechanisms, and pharmacologic properties. Group I includes GRM1 and GRM5 and these receptors have been shown to activate phospholipase C. Group II includes GRM2 and GRM3 while Group III includes GRM4, GRM6, GRM7 and GRM8. Group II and III receptors are linked to the inhibition of the cyclic AMP cascade but differ in their agonist selectivities. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2017] |
| Alternative Names | GLUR2 GPRC1B MGLUR2 mGlu2 4930441L02Rik mGluR7 GRM2_HUMAN GRM2 GRM2_MOUSE GRM2_RAT glutamate metabotropic receptor 2 glutamate receptor metabotropic 2 |
| Molecular Weight(MW) | 96 kDa(Observed band size:＞200 kDa) |
| Cellular Localization | Cell membrane,Cell projection,Membrane,Synapse. |
WB
25 ug total protein per lane of various lysates (see on figure) probed with GRM2 monoclonal antibody, unconjugated at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
IHC
Paraformaldehyde-fixed, paraffin embedded Human Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GRM2 Monoclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by conjugation to HRP‑conjugated secondary antibody and DAB staining.
IHC
Paraformaldehyde-fixed, paraffin embedded Rat Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GRM2 Monoclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by conjugation to HRP‑conjugated secondary antibody and DAB staining.| Application Notes | WB:1:1000-5000 IHC:1:100-500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
