WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-YAP (Phospho Ser397) antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody.| Product Name | YAP (Phospho Ser397) Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Modification | p-Ser397 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG,K |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:2000-1:10000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | YAP1 |
|---|---|
| Gene Synonyms | YAP YKI COB1 YAP2 YAP-1 YAP65 |
| Gene Full Name | Yes1 associated transcriptional regulator |
| Gene Summary | This gene encodes a downstream nuclear effector of the Hippo signaling pathway which is involved in development, growth, repair, and homeostasis. This gene is known to play a role in the development and progression of multiple cancers as a transcriptional regulator of this signaling pathway and may function as a potential target for cancer treatment. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2013] |
| Alternative Names | Yorkie homolog 65 kDa Yes-associated protein YAP65 |
| Molecular Weight(MW) | 54 kDa(Observed band size: 65 kDa) |
| Cellular Localization | Cytoplasm . Nucleus . Both phosphorylation and cell density can regulate its subcellular localization (PubMed:18158288 , PubMed:20048001) . Phosphorylation sequesters it in the cytoplasm by inhibiting its translocation into the nucleus (PubMed:18158288 , PubMed:20048001) . At low density , predominantly nuclear and is translocated to the cytoplasm at high density (PubMed:18158288 , PubMed:20048001 , PubMed:25849865) . PTPN14 induces translocation from the nucleus to the cytoplasm (PubMed:22525271) . Localized mainly to the nucleus in the early stages of embryo development with expression becoming evident in the cytoplasm at the blastocyst and epiblast stages (By similarity) . . |
WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-YAP (Phospho Ser397) antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody.| Application Notes | WB:1:2000-1:10000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year(Do not lower than -25°C). |
| Storage Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA. |
