WB
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CYP2E1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CYP2E1.IHC
Immunohistochemical staining of paraffin-embedded breast tissue within the normal limits using anti-CYP2E1 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.ICC/IF
Anti-CYP2E1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CYP2E1.ICC/IF
Immunofluorescent staining of HepG2 cells using anti-CYP2E1 mouse monoclonal antibody.| Product Name | Cytochrome P450 2E1 (CYP2E1) Mouse Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Full length human recombinant protein of human CYP2E1 (NP_000764) produced in HEK293T cell. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500 IHC:1:50 ICC/IF:1:100 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A/G |
| Gene Symbol | CYP2E1 |
|---|---|
| Gene Synonyms | CPE1 CYP2E P450-J P450C2E |
| Gene Full Name | cytochrome P450 family 2 subfamily E member 1 |
| Gene Summary | This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and is induced by ethanol, the diabetic state, and starvation. The enzyme metabolizes both endogenous substrates, such as ethanol, acetone, and acetal, as well as exogenous substrates including benzene, carbon tetrachloride, ethylene glycol, and nitrosamines which are premutagens found in cigarette smoke. Due to its many substrates, this enzyme may be involved in such varied processes as gluconeogenesis, hepatic cirrhosis, diabetes, and cancer. [provided by RefSeq, Jul 2008] |
| Alternative Names | CPE1 CYP2E P450-J P450C2E |
| Molecular Weight(MW) | 56.8 kDa |
| Cellular Localization | Endoplasmic reticulum,Membrane,Microsome,Mitochondrion,Mitochondrion inner membrane. |
WB
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CYP2E1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CYP2E1.
IHC
Immunohistochemical staining of paraffin-embedded breast tissue within the normal limits using anti-CYP2E1 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.
ICC/IF
Anti-CYP2E1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CYP2E1.
ICC/IF
Immunofluorescent staining of HepG2 cells using anti-CYP2E1 mouse monoclonal antibody.| Application Notes | WB:1:500 IHC:1:50 ICC/IF:1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C as received. |
| Storage Buffer | PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
