WB
Western blot analysis of extracts of various cell lines, using MAPRE1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 10s.ICC/IF
Immunofluorescence analysis of HeLa cells using MAPRE1 antibody at dilution of 1:100 .Blue: DAPI for nuclear staining.| Product Name | MAPRE1 polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant fusion protein of human MAPRE1(NP_036457.1). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| WB:1:500-1:2000 ICC/IF:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MAPRE1 |
|---|---|
| Gene Synonyms | EB1 |
| Gene Full Name | microtubule associated protein RP/EB family member 1 |
| Gene Summary | The protein encoded by this gene was first identified by its binding to the APC protein which is often mutated in familial and sporadic forms of colorectal cancer. This protein localizes to microtubules, especially the growing ends, in interphase cells. During mitosis, the protein is associated with the centrosomes and spindle microtubules. The protein also associates with components of the dynactin complex and the intermediate chain of cytoplasmic dynein. Because of these associations, it is thought that this protein is involved in the regulation of microtubule structures and chromosome stability. This gene is a member of the RP/EB family. [provided by RefSeq, Jul 2008] |
| Alternative Names | MAPRE1 EB1 |
| Molecular Weight(MW) | 34 KDa |
| Cellular Localization | Cell membrane,Cytoplasm,Cytoskeleton,Golgi apparatus,Membrane,Microtubule. |
WB
Western blot analysis of extracts of various cell lines, using MAPRE1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 10s.
ICC/IF
Immunofluorescence analysis of HeLa cells using MAPRE1 antibody at dilution of 1:100 .Blue: DAPI for nuclear staining.| Application Notes | WB:1:500-1:2000 ICC/IF:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. |
| Storage Buffer | 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2. |
