ICC/IF
Immunofluorescence analysis of 3T3 cells labelling Ephrin B2 with Ephrin B2 Rabbit mAb. The cells were fixed with 4% PFA (10min, RT) followed by treatment with 0.1% Triton X-100 (10min, RT), and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with Ephrin B2 Rabbit mAb (1:100) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 (shown in green). Nuclear DNA was labeled in blue with DAPI. Control: Secondary antibody only.| Product Name | Ephrin B2 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthetic peptide of human Ephrin B2. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IF |
| ICC/IF:1:100-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | EFNB2 |
|---|---|
| Gene Synonyms | HTKL EPLG5 Htk-L LERK5 ephrin-B2 |
| Gene Full Name | ephrin B2 |
| Gene Summary | This gene encodes a member of the ephrin (EPH) family. The ephrins and EPH-related receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in mediating developmental events, especially in the nervous system and in erythropoiesis. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. This gene encodes an EFNB class ephrin which binds to the EPHB4 and EPHA3 receptors. [provided by RefSeq, Jul 2008] |
| Alternative Names | HTKL EPLG5 Htk-L LERK5 |
| Molecular Weight(MW) | 37 kDa(Observed band size:45 kDa) |
| Cellular Localization | Cell junction,Cell membrane,Membrane. |
ICC/IF
Immunofluorescence analysis of 3T3 cells labelling Ephrin B2 with Ephrin B2 Rabbit mAb. The cells were fixed with 4% PFA (10min, RT) followed by treatment with 0.1% Triton X-100 (10min, RT), and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with Ephrin B2 Rabbit mAb (1:100) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 (shown in green). Nuclear DNA was labeled in blue with DAPI. Control: Secondary antibody only.| Application Notes | ICC/IF:1:100-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C.Stable for 12 months from date of receipt. |
| Storage Buffer | Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
