WB
Western blot analysis of Catalase on different lysates with Rabbit anti-Catalase antibody at 1/1,000 dilution. Lysates/proteins at 20 µg/Lane. Exposure time: 3 minutes; Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: 1/1,000 in primary antibody dilution buffer, overnight at 4 ℃ Secondary antibody: Anti-Mouse IgG for IP, VHH(HRP), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperatureIHC
Immunohistochemical analysis of paraffin-embedded human liver tissue with Mouse anti-Catalase antibody at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.ICC/IF
ICC staining catalse in HepG2 cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.FC
Flow cytometric analysis of HepG2 cells labeling Catalase. Cells were fixed and permeabilized. Then stained with the primary antibody (1ug/ml) (red) compared with Mouse IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a 488 conjugate-Goat anti-Mouse IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Product Name | Catalase Mouse Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within human Catalase aa 475-527/527. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | FCICC/IFIHCWB |
| WB:1:2000-1:5000 IHC:1:100-1:400 ICC/IF:1:100 FC:1:50 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | CAT |
|---|---|
| Gene Full Name | catalase |
| Gene Summary | This gene encodes catalase, a key antioxidant enzyme in the bodies defense against oxidative stress. Catalase is a heme enzyme that is present in the peroxisome of nearly all aerobic cells. Catalase converts the reactive oxygen species hydrogen peroxide to water and oxygen and thereby mitigates the toxic effects of hydrogen peroxide. Oxidative stress is hypothesized to play a role in the development of many chronic or late-onset diseases such as diabetes, asthma, Alzheimer's disease, systemic lupus erythematosus, rheumatoid arthritis, and cancers. Polymorphisms in this gene have been associated with decreases in catalase activity but, to date, acatalasemia is the only disease known to be caused by this gene. [provided by RefSeq, Oct 2009] |
| Alternative Names | Cas1 antibody CAT antibody CATA_HUMAN antibody Catalase antibody Cs1 antibody MGC138422 antibody MGC138424 antibody |
| Molecular Weight(MW) | 59.8 kDa(Observed band size: 54/60 kDa) |
| Cellular Localization | Peroxisome. |
WB
Western blot analysis of Catalase on different lysates with Rabbit anti-Catalase antibody at 1/1,000 dilution. Lysates/proteins at 20 µg/Lane. Exposure time: 3 minutes; Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: 1/1,000 in primary antibody dilution buffer, overnight at 4 ℃ Secondary antibody: Anti-Mouse IgG for IP, VHH(HRP), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
IHC
Immunohistochemical analysis of paraffin-embedded human liver tissue with Mouse anti-Catalase antibody at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ICC/IF
ICC staining catalse in HepG2 cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
FC
Flow cytometric analysis of HepG2 cells labeling Catalase. Cells were fixed and permeabilized. Then stained with the primary antibody (1ug/ml) (red) compared with Mouse IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a 488 conjugate-Goat anti-Mouse IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Application Notes | WB:1:2000-1:5000 IHC:1:100-1:400 ICC/IF:1:100 FC:1:50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage Buffer | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
