WB
Western Blot. Positive WB detected in: Hela whole cell lysate(20µg), HepG2 whole cell lysate(20µg), 293Twhole cell lysate(20µg), THP-1 whole cell lysate(20µg), MCF7 whole cell lysate(20µg), A431 whole cell lysate(20µg). All lanes: EEF2KMT antibody at 1:1000. Secondary Goat polyclonal to rabbit IgG at 1/20000 dilution. Exposure time: 60s.IHC
IHC image of Rabbit anti-EEF2KMT Polyclonal antibody diluted at 1:100 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody| Product Name | Rabbit anti-EEF2KMT Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthesized peptide derived from human FA86A. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:1000-1:3000 IHC:1:100-1:300 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | EEF2KMT |
|---|---|
| Gene Synonyms | EFM3 SB153 FAM86A eEF2-KMT |
| Gene Full Name | eukaryotic elongation factor 2 lysine methyltransferase |
| Gene Summary | Enables protein-lysine N-methyltransferase activity. Involved in peptidyl-lysine trimethylation. Located in cytoplasm. Part of protein-containing complex. [provided by Alliance of Genome Resources, Jul 2025] |
| Alternative Names | EEF2KMT antibody FAM86A antibody SB153Protein-lysine N-methyltransferase EEF2KMT antibody EC 2.1.1.- antibody eEF2-lysine methyltransferase antibody eEF2-KMT antibody |
| Molecular Weight(MW) | 37, 34 kDa(Observed band size:37 kDa) |
| Cellular Localization | Cytoplasm. |
WB
Western Blot. Positive WB detected in: Hela whole cell lysate(20µg), HepG2 whole cell lysate(20µg), 293Twhole cell lysate(20µg), THP-1 whole cell lysate(20µg), MCF7 whole cell lysate(20µg), A431 whole cell lysate(20µg). All lanes: EEF2KMT antibody at 1:1000. Secondary Goat polyclonal to rabbit IgG at 1/20000 dilution. Exposure time: 60s.
IHC
IHC image of Rabbit anti-EEF2KMT Polyclonal antibody diluted at 1:100 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody| Application Notes | WB:1:1000-1:3000 IHC:1:100-1:300 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Storage Buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
