WB
Relative expression (RE). Western blot analysis of XPC on different lysates with Rabbit anti-XPC antibody at 1/2,000 dilution. Lysates/proteins at 15 µg/Lane. Exposure time: 59 seconds; Low expression：MDA-MB-231 . Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: 1/2,000 in primary antibody dilution buffer, overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP, 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature.IHC
Application: Immunohistochemistry (IHC-P). Species: Human. Tissue: Skin. Sample: Paraffin-embedded section. Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST. Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: 1/1,000, 1 hour at room temperature. Secondary antibody: 20 minutes at room temperature.ICC/IF
Application: Immunocytochemistry (IF-cell) Species: Human Sample: MCF7 (Human breast cancer cell) Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature. Antibody dilution buffer: 1% BSA in PBST. Primary antibody: 1/500, overnight at 4°C. Secondary antibody: Goat Anti-Rabbit IgG (488), 45 minutes at room temperature. Counterstain: Beta tubulin (Red), 1/100, overnight at 4℃. The nuclear counterstain was DAPI (Blue).IP
Immunoprecipitation (IP). XPC was immunoprecipitated in 0.2 mg MCF7 (Human breast cancer cell) cell lysate with XPC Recombinant Rabbit Monoclonal Antibody at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using XPC Recombinant Rabbit Monoclonal Antibody at 1/5,000 dilution. HRP Conjugated Rabbit IgG kappa light chain Mouse Monoclonal Antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: MCF7 cell lysate (input). Lane 2: XPC Recombinant Rabbit Monoclonal Antibody IP in MCF7 cell lysate. Lane 3: Rabbit IgG instead of XPC Recombinant Rabbit Monoclonal Antibody in MCF7 cell lysate. Exposure time: 1 minute 18 seconds. Blocking: 5% NFDM/TBST, 1 hour at room temperature. Primary dilution: 1/5,000 in primary antibody dilution buffer, 2 hours at room temperature.| Product Name | XPC Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within human XPC aa 601-940. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCIPWB |
| WB:1:2000 IHC:1:200-1:1000 ICC/IF:1:500 IP:1-2μg/sample |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | XPC |
|---|---|
| Gene Synonyms | XP3 RAD4 XPCC p125 |
| Gene Full Name | XPC complex subunit, DNA damage recognition and repair factor |
| Gene Summary | The protein encoded by this gene is a key component of the XPC complex, which plays an important role in the early steps of global genome nucleotide excision repair (NER). The encoded protein is important for damage sensing and DNA binding, and shows a preference for single-stranded DNA. Mutations in this gene or some other NER components can result in Xeroderma pigmentosum, a rare autosomal recessive disorder characterized by increased sensitivity to sunlight with the development of carcinomas at an early age. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Aug 2017] |
| Alternative Names | DNA repair protein complementing XP-C cells Xeroderma pigmentosum group C-complementing protein p125 XPC XPCC |
| Molecular Weight(MW) | 106 kDa(Observed band size:120 kDa) |
| Cellular Localization | Nucleus, Chromosome, Cytoplasm. |
WB
Relative expression (RE). Western blot analysis of XPC on different lysates with Rabbit anti-XPC antibody at 1/2,000 dilution. Lysates/proteins at 15 µg/Lane. Exposure time: 59 seconds; Low expression：MDA-MB-231 . Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: 1/2,000 in primary antibody dilution buffer, overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP, 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature.
IHC
Application: Immunohistochemistry (IHC-P). Species: Human. Tissue: Skin. Sample: Paraffin-embedded section. Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST. Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: 1/1,000, 1 hour at room temperature. Secondary antibody: 20 minutes at room temperature.
ICC/IF
Application: Immunocytochemistry (IF-cell) Species: Human Sample: MCF7 (Human breast cancer cell) Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature. Antibody dilution buffer: 1% BSA in PBST. Primary antibody: 1/500, overnight at 4°C. Secondary antibody: Goat Anti-Rabbit IgG (488), 45 minutes at room temperature. Counterstain: Beta tubulin (Red), 1/100, overnight at 4℃. The nuclear counterstain was DAPI (Blue).
IP
Immunoprecipitation (IP). XPC was immunoprecipitated in 0.2 mg MCF7 (Human breast cancer cell) cell lysate with XPC Recombinant Rabbit Monoclonal Antibody at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using XPC Recombinant Rabbit Monoclonal Antibody at 1/5,000 dilution. HRP Conjugated Rabbit IgG kappa light chain Mouse Monoclonal Antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: MCF7 cell lysate (input). Lane 2: XPC Recombinant Rabbit Monoclonal Antibody IP in MCF7 cell lysate. Lane 3: Rabbit IgG instead of XPC Recombinant Rabbit Monoclonal Antibody in MCF7 cell lysate. Exposure time: 1 minute 18 seconds. Blocking: 5% NFDM/TBST, 1 hour at room temperature. Primary dilution: 1/5,000 in primary antibody dilution buffer, 2 hours at room temperature.| Application Notes | WB:1:2000 IHC:1:200-1:1000 ICC/IF:1:500 IP:1-2μg/sample |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage Buffer | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
