WB
Relative expression (RE). Western blot analysis of IL3RA / CD123 on different lysates with Rabbit anti-IL3RA / CD123 antibody at 1/2,000 dilution. Lysates/proteins at 20 µg/Lane. Exposure time: 25 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-IL3RA / CD123 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.ICC/IF
Relative expression (RE). Immunocytochemistry analysis of HUT 102 (positive) and Ramos (negative) labeling IL3RA / CD123 with Rabbit anti-IL3RA / CD123 antibody at 1/250 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-IL3RA / CD123 antibody at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (594) was used as the secondary antibody at 1/1,000 dilution.IF-P
Application: IF-Tissue. Species: Human. Site: B cell lymphoma. Sample: Paraffin-embedded section. Antibody concentration: 1/500.FC
Flow cytometric analysis of human peripheral blood lymphocytes labeling IL3RA / CD123 and CD45RO. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (1/1,000). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Compared with Rabbit IgG Isotype Control (488, 1/1,000).| Product Name | IL3RA / CD123 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within human IL3RA aa 1-325. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIF-PIHCWB |
| WB:1:2000 IHC:1:200-1:1000 ICC/IF:1:250 IF-P:1:500 FC:1:1000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | IL3RA |
|---|---|
| Gene Synonyms | IL3R CD123 IL3RX IL3RY IL3RAY hIL-3Ra IL-3R-alpha |
| Gene Full Name | interleukin 3 receptor subunit alpha |
| Gene Summary | The protein encoded by this gene is an interleukin 3 specific subunit of a heterodimeric cytokine receptor. The receptor is comprised of a ligand specific alpha subunit and a signal transducing beta subunit shared by the receptors for interleukin 3 (IL3), colony stimulating factor 2 (CSF2/GM-CSF), and interleukin 5 (IL5). The binding of this protein to IL3 depends on the beta subunit. The beta subunit is activated by the ligand binding, and is required for the biological activities of IL3. This gene and the gene encoding the colony stimulating factor 2 receptor alpha chain (CSF2RA) form a cytokine receptor gene cluster in a X-Y pseudoautosomal region on chromosomes X or Y. Alternatively spliced transcript variants encoding distinct isoforms have been found. [provided by RefSeq, Jun 2012] |
| Alternative Names | CD123 antibody CD123 antigen antibody hIL 3Ra antibody hIL-3Ra antibody hIL3Ra antibody IL 3 receptor alpha SP2 isoform antibody IL 3R alpha antibody IL-3 receptor subunit alpha antibody IL-3R subunit alpha antibody IL-3R-alpha antibody IL-3RA antibody IL3 Receptor alpha antibody IL3R antibody Il3ra antibody IL3RA_HUMAN antibody IL3RAX antibody IL3RAY antibody IL3RX antibody IL3RY antibody Interleukin 3 receptor alpha chain antibody Interleukin 3 receptor alpha (low affinity) antibody Interleukin 3 receptor alpha antibody Interleukin-3 Receptor alpha antibody Interleukin-3 receptor subunit alpha antibody Interleukin3 receptor antibody Interleukin3 receptor Y-chromosomal antibody |
| Molecular Weight(MW) | 43 kDa(Observed band size:70 kDa) |
| Cellular Localization | Membrane. |
WB
Relative expression (RE). Western blot analysis of IL3RA / CD123 on different lysates with Rabbit anti-IL3RA / CD123 antibody at 1/2,000 dilution. Lysates/proteins at 20 µg/Lane. Exposure time: 25 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-IL3RA / CD123 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ICC/IF
Relative expression (RE). Immunocytochemistry analysis of HUT 102 (positive) and Ramos (negative) labeling IL3RA / CD123 with Rabbit anti-IL3RA / CD123 antibody at 1/250 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-IL3RA / CD123 antibody at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (594) was used as the secondary antibody at 1/1,000 dilution.
IF-P
Application: IF-Tissue. Species: Human. Site: B cell lymphoma. Sample: Paraffin-embedded section. Antibody concentration: 1/500.
FC
Flow cytometric analysis of human peripheral blood lymphocytes labeling IL3RA / CD123 and CD45RO. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (1/1,000). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Compared with Rabbit IgG Isotype Control (488, 1/1,000).| Application Notes | WB:1:2000 IHC:1:200-1:1000 ICC/IF:1:250 IF-P:1:500 FC:1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
