WB
WB analysis of HeLa using CLTC Monoclonal antibody. HeLa, Jurkat, ROS1728 cells, and pig brain tissue were subjected to SDS PAGE followed by western blot with CLTC antibody at dilution of 1:40000 incubated at room temperature for 1.5 hours.WB
WB analysis of Raji using CLTC Monoclonal antibody. Raji cells were subjected to SDS PAGE followed by western blot with CLTC antibody at dilution of 1:40000 incubated at room temperature for 1.5 hours.WB
WB analysis using CLTC Monoclonal antibody. Various lysates were subjected to SDS PAGE followed by western blot with CLTC antibody at dilution of 1:50000 incubated at room temperature for 1.5 hours.IHC
IHC staining of human breast cancer using CLTC Monoclonal antibody. Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using CLTC antibody at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).IHC
IHC staining of mouse kidney using CLTC Monoclonal antibody. Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using CLTC antibody at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).ICC/IF
IF Staining of HeLa using CLTC Monoclonal antibody. Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CLTC antibody, at dilution of 1:600 and 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).| Product Name | CLTC Monoclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human CLTC protein. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHCWB |
| WB:1:20000-1:100000 IHC:1:1000-1:4000 ICC/IF:1:400-1:1600 |
|
| Species Reactivity | HumanMousePigRabbitRat |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Gene Symbol | CLTC |
|---|---|
| Gene Synonyms | Hc CHC CHC17 MRD56 CLH-17 CLTCL2 |
| Gene Full Name | clathrin heavy chain |
| Gene Summary | Clathrin is a major protein component of the cytoplasmic face of intracellular organelles, called coated vesicles and coated pits. These specialized organelles are involved in the intracellular trafficking of receptors and endocytosis of a variety of macromolecules. The basic subunit of the clathrin coat is composed of three heavy chains and three light chains. [provided by RefSeq, Jul 2008] |
| Alternative Names | 1B3D7 CHC CHC17 Clathrin heavy chain Clathrin heavy chain 1 |
| Molecular Weight(MW) | 192 kDa(Observed band size:170 kDa) |
| Cellular Localization | Coated pit,Cytoplasm,Cytoplasmic vesicle,Cytoskeleton,Membrane. |
WB
WB analysis of HeLa using CLTC Monoclonal antibody. HeLa, Jurkat, ROS1728 cells, and pig brain tissue were subjected to SDS PAGE followed by western blot with CLTC antibody at dilution of 1:40000 incubated at room temperature for 1.5 hours.
WB
WB analysis of Raji using CLTC Monoclonal antibody. Raji cells were subjected to SDS PAGE followed by western blot with CLTC antibody at dilution of 1:40000 incubated at room temperature for 1.5 hours.
WB
WB analysis using CLTC Monoclonal antibody. Various lysates were subjected to SDS PAGE followed by western blot with CLTC antibody at dilution of 1:50000 incubated at room temperature for 1.5 hours.
IHC
IHC staining of human breast cancer using CLTC Monoclonal antibody. Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using CLTC antibody at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC
IHC staining of mouse kidney using CLTC Monoclonal antibody. Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using CLTC antibody at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
ICC/IF
IF Staining of HeLa using CLTC Monoclonal antibody. Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CLTC antibody, at dilution of 1:600 and 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).| Application Notes | WB:1:20000-1:100000 IHC:1:1000-1:4000 ICC/IF:1:400-1:1600 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
