WB
Western blot analysis of FLAP/ALOX5AP using anti-FLAP/ALOX5AP antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-FLAP/ALOX5AP antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.IHC
HC analysis of FLAP/ALOX5AP using anti-FLAP/ALOX5AP antibody. FLAP/ALOX5AP was detected in a paraffin-embedded section of human tonsil tissue. The tissue section was incubated with rabbit anti-FLAP/ALOX5AP Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.ICC/IF
ICC/IF analysis of FLAP/ALOX5AP using anti-FLAP/ALOX5AP antibody. FLAP/ALOX5AP was detected in an immunocytochemical section of U2OS cells. The section was incubated with rabbit anti-FLAP/ALOX5AP Antibody at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody. The section was counterstained with DAPI (Blue).FC
Flow Cytometry analysis of Jurkat cells using anti-FLAP/ALOX5AP antibody. Overlay histogram showing Jurkat cells stained with Rabbit polyclonal antibody to FLAP/ALOX5AP (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-FLAP/ALOX5AP Antibody Rabbit polyclonal antibody to FLAP/ALOX5AP at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG Rabbit polyclonal antibody to FLAP/ALOX5AP was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Product Name | Rabbit polyclonal antibody to FLAP/ALOX5AP |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human ALOX5AP, identical to the related mouse and rat sequences. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 FC:1:50-1:200 |
|
| Species Reactivity | Human |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ALOX5AP |
|---|---|
| Gene Synonyms | FLAP |
| Gene Full Name | arachidonate 5-lipoxygenase activating protein |
| Gene Summary | This gene encodes a protein which, with 5-lipoxygenase, is required for leukotriene synthesis. Leukotrienes are arachidonic acid metabolites which have been implicated in various types of inflammatory responses, including asthma, arthritis and psoriasis. This protein localizes to the plasma membrane. Inhibitors of its function impede translocation of 5-lipoxygenase from the cytoplasm to the cell membrane and inhibit 5-lipoxygenase activation. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Feb 2011] |
| Alternative Names | MK-886-binding protein ALOX 5AP ALOX5 AP ALOX5AP FLAP |
| Molecular Weight(MW) | 18 kDa |
| Cellular Localization | Endoplasmic reticulum,Membrane,Nucleus. |
WB
Western blot analysis of FLAP/ALOX5AP using anti-FLAP/ALOX5AP antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-FLAP/ALOX5AP antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
IHC
HC analysis of FLAP/ALOX5AP using anti-FLAP/ALOX5AP antibody. FLAP/ALOX5AP was detected in a paraffin-embedded section of human tonsil tissue. The tissue section was incubated with rabbit anti-FLAP/ALOX5AP Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
ICC/IF
ICC/IF analysis of FLAP/ALOX5AP using anti-FLAP/ALOX5AP antibody. FLAP/ALOX5AP was detected in an immunocytochemical section of U2OS cells. The section was incubated with rabbit anti-FLAP/ALOX5AP Antibody at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody. The section was counterstained with DAPI (Blue).
FC
Flow Cytometry analysis of Jurkat cells using anti-FLAP/ALOX5AP antibody. Overlay histogram showing Jurkat cells stained with Rabbit polyclonal antibody to FLAP/ALOX5AP (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-FLAP/ALOX5AP Antibody Rabbit polyclonal antibody to FLAP/ALOX5AP at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG Rabbit polyclonal antibody to FLAP/ALOX5AP was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 FC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt，-20℃ as supplied. |
| Storage Buffer | 500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
