WB
Western blot analysis of lysates from Rat kidney, using NELL1 Antibody. The lane on the left was treated with blocking peptide.ICC/IF
Rabbit polyclonal antibody to NELL1 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(1:200) and mouse anti-beta tubulin Ab(1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI(blue).| Product Name | Rabbit polyclonal antibody to NELL1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthesized peptide derived from human NELL1, corresponding to a region within the internal amino acids. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| WB:1:500-1:2000 ICC/IF:1:100-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NELL1 |
|---|---|
| Gene Synonyms | IDH3GL |
| Gene Full Name | neural EGFL like 1 |
| Gene Summary | This gene encodes a cytoplasmic protein that contains epidermal growth factor (EGF)-like repeats. The encoded heterotrimeric protein may be involved in cell growth regulation and differentiation. A similar protein in rodents is involved in craniosynostosis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2013] |
| Alternative Names | FLJ45906 IDH3GL NEL like 1 (chicken) NEL like protein 1 Nel related protein 1 NEL-like 1 (chicken) NEL-like protein 1 Nel-related protein 1 NELL 1 protein short isoform Nell1 NELL1_HUMAN Neural epidermal growth factor like 1 NRP1 Protein kinase C binding protein NELL1 Protein kinase C-binding protein NELL1 |
| Molecular Weight(MW) | 90 kDa |
| Cellular Localization | Cytoplasm. Nucleus envelope. Secreted. |
WB
Western blot analysis of lysates from Rat kidney, using NELL1 Antibody. The lane on the left was treated with blocking peptide.
ICC/IF
Rabbit polyclonal antibody to NELL1 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(1:200) and mouse anti-beta tubulin Ab(1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI(blue).| Application Notes | WB:1:500-1:2000 ICC/IF:1:100-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 °C. Stable for 12 months from date of receipt. |
| Storage Buffer | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
